Akagi, Takashi, Isabelle M. Henry, Takashi Kawai, Luca Comai, and Ryutaro Tao. ‘Epigenetic Regulation of the Sex Determination GeneMeGIin Polyploid Persimmon’.The Plant Cell28, no. 12 (December 2016): 2905–15.https://doi.org/10.1105/tpc.16.00532.
中文:
我们着手研究柿子的性别决定机制,尤其是我们想要噶清楚为何基因型为雄性的树可以同时产生雄花和雌花。两个物种(六倍体柿子和二倍体柿子)的花发育和大多数性别决定分子机制都是保守的,但我们也发现了两点显著不同。第一,可能是由于其启动子区域SINE-like 元素的插入,在D. kaki的发育中的雄花检测不到OGI的表达;第二,我们发现性别决定和MeGI启动子甲基化密切相关,具体表现在发育为雄花的花芽中有5'胞嘧啶甲基化并抑制MeGI的表达,但在其他植物组织和发育为雌花的花芽中没有这个现象。为了验证这个猜想,我们观察到使用甲基化抑制剂处理正在发育中的雄花可产生雌化的花。
英文原文:
We set out to investigate the mechanism underlying sex determination in persimmons; more specifically, we wanted to understand how a tree that is genetically male can produce both male and female flowers. While flower development and most of the molecular pathway are conserved between the two species, we found two notable differences. First, OGI expression remained undetectable in developing male flowers of D.kaki, potentially due to the insertion of a SINE-like element in its promoter. Second, we found a tight association between sex determination and methylation of MeGI promoter, which exhibits 5'cytosine methylation and repression of MeGI expression in buds that form male flowers, but not in the rest of the plant body or in buds that form female flowers. Confirming this hypothesis, we observed that treatment of developing male flower with a methylation inhibitor could result in feminized flowers.
这段也是结构很好,逻辑很清晰。
首先第一句:我们想要研究的现象:基因型为雄的树上开雌花和雄花;
第二句:比较本次研究物种与2014年science研究物种的异同,先同再异,从一般到特殊;
第三、四句:我们所发现的具体两点不同以及我们的推论;
最后一句:为了验证以上猜想我们所做的实验及结果。
这段是讨论的第一段,但其实已经概括了整篇文章的背景,发现及重点结论,非常凝练,逻辑流畅。
在比较不同组织中甲基化情况时,in buds that form xx flowers出现了两次,因为中间有其他内容,所以也并不显得重复,我觉得应该算是不错的平行结构。
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