Q:怎么用双突变体确认两个基因的上下游关系?
A:双突变体(A+B)为单突变体A的表型,则基因B在上游。总开关是下游。
起因:读文献有一句话我不能理解
"pif3-1 ebf1-1 double mutant behaved like pif3-1 single mutant in terms of freezing tolerance and ion leakage. The expression of CBFs and their target genes in ebf1-1 was partially rescued by the pif3-1 mutant either after cold treatment or during the nighttime at warm temperatures. These results demonstrate the PIF3 acts downstream of EBF1 to regulate the CBF pathway and freezing tolerance.
文献涞源:PIF3 is a negative regulator of the CBF pathway and freezing tolerance in Arabidopsis
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