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网站预测结果：
A domains aligned with results of PONDR (predictor of natural disordered regions) and NCPR (net charge per residue; 5-amino acid window) analyses.
免疫荧光结果：
G3BP1/2 dKO cells were transfected with indicated G3BP1 constructs, exposed to 某种处理因素 (浓度/剂量; 时间), and stained for the SG marker eIF3h染（色蛋白）. Scale bar, 20 mm.
相分离拍摄结果：
LLPS of purified recombinant G3BP1 in 150 mM NaCl and 50 ng/mL total RNA and the absence of a crowding agent. Scale bar, 10 mm.
LLPS of purified recombinant G3BP1 with 100 ng/mL total RNA, with or without the addition of 1 mg/mL RNase A. Scale bar, 50 mm.
GraphPad统计图：
Cells from (C) were imaged, and the percentage of cells positive for SGs was quantified. Error bars indicate SD. ****p < 0.0001 versus NC by one-way ANOVA; Dunnett’s multiple comparisons test.
The percentage of cells positive for SGs was quantified (E). Scale bars, 50 mm. Error bars indicate SD. **p = 0.0098 versus G3BP1 by two-way ANOVA; Sidak’s multiple comparisons test.
免疫共沉淀：
Immunoprecipitated G3BP1 cross-linked to RNA was assessed by immunoblotting for biotin (RNA) and GFP (G3BP1). Binding of RNA was quantified and normalized to protein signal. Results show quantification of triplicate experiments. Error bars indicate SD. ****p < 0.0001 versus NC by one-way ANOVA; Dunnett’s multiple comparisons test.
蛋白质3D结构展示：
Structures of G3BP1 NTF2L (PDB: 4FCJ), GST (PDB: 1UA5), and FKBPF36M (PDB: 1YEM) dimers.
生信分析结果：
KEGG pathway analyses showing the top ten pathways affected by AFAP1-AS1.
Hierarchical cluster plot of the differentially expressed and metastasis-associated genes between H1299-control or H1299-AFAP1-AS1 cells, using a cutoff of fold change > 2 and adjusted p value < 0.05.
GO analyses listing the top ten biological processes regulated by AFAP1-AS1.
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