Bowtie: Error: reads file does n

使用Bowtie进行短序列比对，代码如下

nohup bowtie -q -v 2 -l 10 -k 1 /zfssz3/ST_SUPERCELLS/USER/zhangdengwei/miRBase/human/02.bowtie_index/mature.hsa_U_to_T ../../1.fastp/SRR522851/SRR522851_trimmed.fq.gz -S align.sam > mapping.log 2>&1 &

报错

nohup: ignoring input
Error: reads file does not look like a FASTQ file
terminate called after throwing an instance of 'int'

查看参数发现其中-q参数为

  -q                 query input files are FASTQ .fq/.fastq (default)

并未说是.fq.gz，将原始文件进行解压再测试

nohup bowtie -q -v 2 -l 10 -k 1 /zfssz3/ST_SUPERCELLS/USER/zhangdengwei/miRBase/human/02.bowtie_index/mature.hsa_U_to_T ../../1.fastp/SRR522851/SRR522851_trimmed.fq -S align.sam > mapping.log 2>&1 &

果然可以了，巨坑