nt/nr物种 | megan/Blast2lca

Github:
https://github.com/emepyc/Blast2lca
https://github.com/husonlab/megan-ce

Download installers for MEGAN Community Edition here:
http://www-ab.informatik.uni-tuebingen.de/software/megan6

运行

tool="/ldfssz1/ST_DIVERSITY/PUB/USER/zhouchengran/software/Meta/Megan/megan/tools"
$tool/blast2lca \
-i demo_shangjin/assembling_MT_1_1.200.noaster.faa.1.filter.blastp \
-o out \
-f BlastTab -mdb megan6/megan-map-Jan2021.db

过程

Version   MEGAN Community Edition (version 6.21.16, built 28 Oct 2021)
Author(s) Daniel H. Huson
Copyright (C) 2021 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY.
Loading ncbi.map: 2,302,807
Loading ncbi.tre: 2,302,811
Loading kegg.map:   100,399
Loading kegg.tre:   106,785
Reading file: demo_shangjin/assembling_MT_1_1.200.noaster.faa.1.filter.blastp
Writing file: out
Processing file: demo_shangjin/assembling_MT_1_1.200.noaster.faa.1.filter.blastp
10% 10% 20% 20% 30% 30% 40% 40% 50% 50% 60% 60% 70% 70% 80% 80% 90% 90% 100% (557.1s)
100% (557.1s)
Reads in:         269
Reads out:        269
Time: 573s

参考：
Using AnnoTree to Get More Assignments, Faster, in DIAMOND+MEGAN Microbiome Analysis ASM Journals mSystems Vol. 7 No1 (22.2.2022)