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PART1 m6A/MeRIP-seq

PART1 m6A/MeRIP-seq

作者: 小qqq | 来源:发表于2022-07-01 23:41 被阅读0次

    此次学习的m6A/MeRIP-seq 适合单个细胞样本或者bulk样本

    work flow
    image.png

    需要用到的包:

    Cutadapt, tested with 1.15
    Samtools, tested with 1.7
    HISAT2, tested with 2.1.0
    Picard, tested with 2.17.10
    bedtools, tested with 2.27.1
    bedGraphToBigWig
    StringTie, tested with 1.3.4d
    prepDE.py
    gffcompare, tested with 0.10.4
    MACS2, tested with 2.1.1.20160309
    HOMER, tested with 4.9
    R, tested with 3.3.3. Install three libraries exomePeak, MeTPeak and MeTDiff in R.

    参考文档:shunliubio/easym6A: Process m6A/MeRIP-seq data in a single or batch job mode (github.com)

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