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StringTie定量出现COV负值报错

StringTie定量出现COV负值报错

作者: 信你个鬼 | 来源:发表于2021-02-14 22:32 被阅读0次

    出现如下错误:


    image.png

    检查对应样本,出现负值:


    image.png
    14  StringTie   transcript  50329404    50396912    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; ref_gene_name "ENSG00000100490"; cov "-2.600490"; FPKM "0.000000"; TPM "0.000000";
    14  StringTie   exon    50329404    50330181    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "1"; ref_gene_name "ENSG00000100490"; cov "-4.341874";
    14  StringTie   exon    50334565    50334621    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "2"; ref_gene_name "ENSG00000100490"; cov "-7.871197";
    14  StringTie   exon    50338947    50339029    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "3"; ref_gene_name "ENSG00000100490"; cov "-4.112955";
    14  StringTie   exon    50341032    50341232    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "4"; ref_gene_name "ENSG00000100490"; cov "-2.416990";
    14  StringTie   exon    50342132    50342222    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "5"; ref_gene_name "ENSG00000100490"; cov "-1.085523";
    14  StringTie   exon    50344986    50345058    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "6"; ref_gene_name "ENSG00000100490"; cov "-0.774802";
    14  StringTie   exon    50359028    50359149    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "7"; ref_gene_name "ENSG00000100490"; cov "-0.659754";
    14  StringTie   exon    50395701    50396329    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "8"; ref_gene_name "ENSG00000100490"; cov "-0.774150";
    14  StringTie   exon    50396824    50396912    1000    -   .   gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "9"; ref_gene_name "ENSG00000100490"; cov "-1.620675";
    

    然后查到是一个bug,还被反复修来修去:


    image.png image.png

    根据报错信息:stringtie negative 能检索到更多信息。

    额,但是还不清楚这个软件计算cov咋计算的。

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