2022.11.08

Yang, Tao. ‘Improved Pea Reference Genome and Pan-Genome Highlight Genomic Features and Evolutionary Characteristics’.Nature Genetics54 (2022): 18.

Abstract

Zicola, Johan. ‘MITE Not Be That Bad after All: A Transposon Promoting Drought Resistance in Apple’.The Plant Cell34, no. 10 (27 September 2022): 3505–6.https://doi.org/10.1093/plcell/koac231.

实验流程

中文：

仔细观察MdRFNR1的两个等位基因，研究者们鉴定到一个430bp的微型反向重复转座子插入到了MdRNFR1-1的启动子上。他们通过PCR发现这个MITE在371个Malus种质中的30个上都存在。作者随后观察了两个等位基因基因间的表达量差异，发现Md在根中高表达，并且可以在试管中被干旱压力诱导。为了检验MITE插入是否调控干旱压力介导的MdRFNR1表达，他们在苹果愈伤组织转入MITE插入与未插入的MdRNFR1-1启动子转录的GUS报告基因。结果显示只有在MITE存在的情况下GUS表达会被干旱诱导，证实了它在顺势调控中的作用。

英文：

Looking carefully at the two alleles of MdRFNR1, the reaserchers identified a 430bp miniature inverted-repeat TE insertion in the promoter of MdRFNR1-1. They found this MITE insertion in 30 out of 371  Malus accessions genotyped by PCR. They then looked at the expression difference between the two alleles and found that MdRFNR1-1 is more highly expressed in the root and could be further induced by drought stress in vitro. To test whether the MITE insertion mediates drought stress induction of MdRFNR1 expression, they transformed apple calli with a GUS reporter gene driven by the promoter of MdRFNR1-1 with and without the MITE insertion. They showed that GUS expression is induced by drought stress only when the MITE insertion is present, confirming its role in cis-regulation.

一段话概括了原文作者的工作，凝练，逻辑顺畅。

1.looking at the gene structure of two alleles: found a MITE insertion in MdRFNR1-1；

2.looking at the gene expression of two alleles: found specific expression of MdRFNR1-1;

3. testing the hypothetic relationship of 1 & 2;

4. confirming the relationship: MITE insertion cis regulate MdRFNR1-1.