转载自 马疾香幽的博客
Fieldsmustbetab-separated. Also, all but the final field in each feature linemust contain a value; "empty" columns should be denoted with a'.'
seqname- name of the chromosomeor scaffold; chromosome names can be given with or without the'chr' prefix.Important note: the seqnamemust be one used within Ensembl, i.e. a standard chromosome name oran Ensembl identifier such as a scaffold ID, without any additionalcontent such as species or assembly. See the example GFF outputbelow.
source- name of the program thatgenerated this feature, or the data source (database or projectname)
feature- feature type name, e.g.Gene, Variation, Similarity
start- Start position of thefeature, with sequence numbering starting at 1.
end- End position of thefeature, with sequence numbering starting at 1.
score- A floating pointvalue.
strand- defined as + (forward)or - (reverse).
frame- One of '0', '1' or '2'.'0' indicates that the first base of the feature is the first baseof a codon, '1' that the second base is the first base of a codon,and so on..
attribute- A semicolon-separatedlist of tag-value pairs, providing additional information abouteach feature.
1.染色体名
2.注释信息的来源，比如”Genescan”、”Genbank”
等，可以为空，为空用”.”点号代替
3.注释信息的类型，比如Gene、cDNA、mRNA等，或者是SO对应的编号
4、5.开始和结束位置
6.得分，数字，是注释信息可能性的说明，可以是序列相似性比对时的E-values值或者基因预测是的P-values值。”.”表示为空。
7.序列的方向，
+表示正义链, -反义链 , ? 表示未知
8.阅读框：有数字0、1和2。0代表序列的第一个碱基为密码子的第一个碱基，1代表是密码子第二个，2代表第三个。
9.以多个键值对组成的注释信息描述，键与值之间用”=“，不同的键值用”;“隔开，一个键可以有多个值，不同值用”,“分割。注意如果描述中包括tab键以及”,=;”，要用URL转义规则进行转义，如tab键用
代替。键是区分大小写的，以大写字母开头的键是预先定义好的，在后面可能被其他注释信息所调用
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