targetp

这个工具有个bt的地方在于，每个文件的residue总数不能超过20w。
所以要是结果不太正常，那就实施输入少数的数目会是什么情况，结果发现，我们的residue数目是有限制的。。
targetp的README居然也没有提到这一点。 。。。太坑乐
Input exceeded maximal total residues : 200000

因此我的处理办法便是，让50个文件都

安装targetp之后，有以下说明都要按照上面一一写上：
uname (usually /bin/uname)
paste ( /bin/paste)
perl ( /usr/bin/perl)
nawk (or equivalent, e.g. gawk)
sh (usually /bin/sh)
echo ( /bin/echo)

1. Decide where you wish to keep the software. Uncompress and untar the
   package in that location:

   cat TargetP-1.1.tar.Z | uncompress | tar xvf -

   This will produce a directory 'TargetP-1.1'. The size of the installed
   package is at most 7 Mb, depending on which binaries are included.
   这里尤其要注意awk executable，ubuntu 15.0不要用awk文件，要
   sudo apt-get install gawk
   来下载gawk，并赋予gawk的路径！

2. Edit the paragraph labelled "GENERAL SETTINGS, CUSTOMIZE" at the top of
   the file "targetp". Configure the following variables:

         Variable        Set to the full path to:
         -------------------------------------------
         TARGETP         'targetp-1.1' directory
         TMP             location for temporary data
         PASTE           'paste' executable
         PERL            'perl' executable
         AWK             'awk' executable
         SH              POSIX-compliant shell
         ECHO            'echo' executable
         -------------------------------------------
   

   Make sure that the settings you enter point at existing and operational
   utilities. You might need to modify them after the testing (see below).

3. Edit the paragraph labelled "HELPER APPLICATIONS, CUSTOMIZE" further
   down in the same file. Configure the following variables:

         Variable        Set to the full path to:
         ----------------------------------------
         CHLOROP         'chlorop' executable
         SIGNALP         'signalp' executable
   

   TargetP will need those softwares to produce the output as described in
   the reference quoted above. They will be employed if the '-c' option is
   used but not otherwise. The decision whether to install them should be
   with the future users of TargetP:

./targetp -P test/twelve.fsa # many sequences