运行STAR时遇到的错误

在开始对比时，使用如下参数

STAR \
    --genomeDir STAR \
    --runThreadN 39 \
    --readFilesIn /raid1/wzw/Ensete/hisat/Ensete_RNA_1.fq /raid1/wzw/Ensete/hisat/Ensete_RNA_2.fq \
    --readFilesType Fastx\
    --outFileNamePrefix ECLrna_ \
    --outSAMtype BAM SortedByCoordinate \
    --outBAMsortingThreadN 39 \
    --outSAMstrandField intronMotif \
    --outFilterIntronMotifs RemoveNoncanonical

出现如下错误
BAMoutput.cpp:27:BAMoutput: exiting because of OUTPUT FILE error: could not create output file ECLrna_STARtmp//BAMsort/8/80
SOLUTION: check that the path exists and you have write permission for this file. Also check ulimit -n and increase it to allow more open files.

阅读错误发现应该是bamsort的时候会使用大量进程，导致超过了最大线程数调整--outBAMsortingThreadN 参数到5

--outBAMsortingThreadN 39 

终于正常运行了