题目: Chemo-resistant Gastric Cancer Associated Gene Expression Signature: Bioinformatics Analysis Based on Gene Expression Omnibus
DOI: 10.21873/anticanres.13274
数据集: GSE31811,GSE26253
样本:
GSE31811: 8 non-responders, 11 early-responders
GSE26253: 432
分析方法:
DEG: Agilent 2100 Bioanalyzer (p<0.05 and |log2FC| ≥1)
DAVID: GO和KEGG富集分析
Cytoscape: PPI
GEPIA: survival analysis
文章数据：
-
分析流程
2.差异上下调基因GO和pathway分析
3.PPI网络分析
找出hub基因(Hub genes including GNG7,PLCB1, CALML5, FGFR4, GRB2, JAK3, ADCY7, ADCY9, GNAS and KDR)
4 hub基因在GSE26253,TCGA中验证
网友评论