Defining the Teratoma as a Model for Multi-lineage Human Development

数据： 179,632 cells across 23 teratomas from 4 cell lines
细胞类型： approximately 20 cell types across all 3 germ layers
观点：单细胞实验20种细胞类型，和类器官和胚胎的数据做比较，利用CRISPR-Cas9做敲除扫描。利用miRNA调节的自杀基因来塑造特定谱系的teratomas

Introduction

1.人类发育模型的受限
2.利用ESC或者iPSC的分化出现在二维空间
3.3D的类器官的细胞类型往往是immature
4.利用teratoma的优势
5.teratoma的研究过程
6.研究内容

Result

Teratoma Characterization
Assaying Teratoma Heterogeneity
Engineering Teratomas via Genetic Perturbations
Modeling Neural Disorders Using Teratomas
Engineering Teratomas via miRNA-Based Molecular
Sculpting

Single-cell transcriptional diversity is a hallmark of developmental potential

1.RNA-based correlates of single-cell differentiation states

2. Development of CytoTRACE
3. Performance evaluation across tissues, species, and platforms
4. Stem cell–related genes and hierarchies
5. Application to neoplastic disease