Pan-cancer characterization of i

题目：免疫相关LncRNA在啊泛癌中的特征提取进而识别癌症标志物

核心内容：

1. ImmLnc pipeline, for identifying lncRNA regulators of immune-related pathways
2. landscape of lncRNA regulation in the immunome across 33 cancer types
3. The immune-related lncRNAs are likely to show expression perturbation in cancer and are significantly correlated with immune cell infiltration
4. identify three molecular subtypes (proliferative, intermediate, and immunological) of non-small cell lung cancer
5. a valuable resource for understanding lncRNA function

免疫相关词汇和数据

1）The cytokine（细胞因子） and chemokine （趋化因子）system 是免疫治疗的潜在靶点
2） 17 immunologically relevant gene sets representing distinct immune pathways derived from ImmPort
3）“IFNG_score” （干扰素γ）and “Cytotoxic cells”（细胞毒性细胞） score
4）Six sample-matched gene and lncRNA expression profiles from The International Cancer Genome Consortium 22 and Gene Expression Omnibus.
5） Ten single-cell sequencing data for immune cells downloaded from PanglaoDB
6） Rhe lncRNA expression profile in immune cells of TCGA bulk RNA sequencing (RNA-Seq) samples based on the ideas from RESPECTEx
7）We next estimated the immune cell infiltration levels of each
patient based on gene expression by Tumor Immune Estimation
Resource (TIMER) and CIBERSORT
8）The tumor mutation burden (TMB) is emerging as a potential
biomarker for immunotherapy.
9）homologous recombination deficiency (HRD)(同源重组缺乏征) remains an important bio-marker and a potentially effective adjunct to enhance immunogenicity of tumors.
10）immune cytolytic activity (CYT) and major histocompatibility complex (MHC) scores[免疫细胞溶解活性（CYT）和主要组织相容性复合物（MHC）评分]

Result

1）Identification of immune-related lncRNAs across cancer types.

识别方法

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2）Expression perturbation of lncRNA regulators in cancer.

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3）LncRNA are associated with immune cell infiltration

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4）ImmLnc prioritizes cancer-related lncRNAs

方法：For each lincRNA, we obtained 17 relative ranks based on the immune pathways. Next, we also ranked the lincRNAs based on the number of cancer types in which they showed expression perturbations. Finally, the lincRNAs were ranked based on the average of 18 relative RSs 图片.png

where R i is the normalized rank based on the immune pathway and R deg is the normalized rank based on the differential expression analysis.

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5）Immune lncRNAs classify molecular subtypes of cancer.

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6）Immune profiling suggests existence of immunology subtype

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7）ImmLnc: a web-based resource for immune lncRNAs in cancer.

ImmLnc web resource (http://bio-bigdata.hrbmu.edu.cn/ImmLnc)