HepG2细胞系

Derivation

HepG2 was derived from a liver hepatocellular carcinoma of a 15 year old Caucasian male.
HepG2来源于一名15岁白人男性的肝癌细胞。

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Receptor Expression

insulin; insulin-like growth factor II (IGF II)

Genes Expressed

alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen;complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein)
α-甲胎蛋白;白蛋白;alpha2巨球蛋白;α1抗胰蛋白酶;转铁蛋白;α1抗胰凝乳蛋白酶;结合珠蛋白;血浆铜蓝蛋白;纤溶酶原;物,complement(C4);C3活化剂;纤维蛋白原;α1酸性糖蛋白;α2 HS糖蛋白;β脂蛋白;视黄醇结合蛋白。
Comments
The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities.
细胞表达3-羟基-3-甲基戊二酰辅酶a还原酶和肝甘油三酯脂肪酶活性。
The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress).
细胞在氧化应激反应中apoA-I mRNA表达降低，过氧化氢酶mRNA表达升高。
There is no evidence of a Hepatitis B virus genome in this cell line.
在这个细胞系中没有乙肝病毒基因组的证据。
In addition to the hepatocellular carcinoma or hepatoma cell line based on the original publication (PubMed: 6248960), HepG2 is also referred as hepatoblastoma cell line (PubMed: 19751877).
除了原发表的肝细胞癌或肝癌细胞系(PubMed: 6248960)外，HepG2也被称为肝母细胞瘤细胞系(PubMed: 19751877)。

Complete Growth Medium

完全生长培养基
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
该细胞系的基本培养基是ATCC配制的Eagle's Minimum Essential培养基，目录号30-2003。要制作完整的生长培养基，在基础培养基中添加以下成分：终浓度为10%的胎牛血清。

Subculturing

传代培养
Volumes are given for a 75 cm2 flask. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
75平方厘米的培养瓶。推荐Corning®培养瓶（目录430641）用于该产品的继代培养。按比例增加或减少其他大小培养血管所需的分离培养基的数量。

1.Remove and discard culture medium.

弃去培养基。

2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

用0.25%（w/v）胰蛋白酶-0.53mm EDTA溶液短暂冲洗细胞层，去除所有含有胰蛋白酶抑制剂的血清。

3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

向烧瓶中加入2.0至3.0毫升胰蛋白酶EDTA溶液，在倒置显微镜下观察细胞，直到细胞层分散（通常在5至15分钟内）。
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
注意：为避免结块，在等待细胞分离时，不要通过敲击或摇晃烧瓶来搅动细胞。难以分离的细胞可放置在37°C以便于分散。

4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

加入6.0至8.0毫升的完全生长培养基，用移液管轻轻吸出细胞。

5.Add appropriate aliquots of the cell suspension to new culture vessels.

在新的培养血管中加入适量的细胞悬液。

6.Incubate cultures at 37°C.

在37℃下培养。
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
传代比：建议继代比为1:4至1:6
培养基更换：每周两次