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测序质控软件-trim_galore安装(2018-05-25)

测序质控软件-trim_galore安装(2018-05-25)

作者: 简单点lili | 来源:发表于2018-05-25 13:48 被阅读380次

    1. 源代码下载:https://github.com/FelixKrueger/TrimGalore

    2. 查看安装日志:

    Installation

    Trim Galore!is a a Perl wrapper around two tools: Cutadapt and FastQC. To use, ensure that these two pieces of software are available and copy the trim_galore script to a location available on the PATH.

    For example:

    #Check that cutadapt is installed

    cutadapt --version

    #Check that FastQC is installed

    fastqc -v

    #Install Trim Galore!

    curl -fsSL https://github.com/FelixKrueger/TrimGalore/archive/0.4.5.tar.gz -o trim_galore.tar.gz tar xvzf trim_galore.tar.gz

    mv TrimGalore-0.4.5/trim_galore /usr/bin

    大概是说安装这个软件上之前需要安装Fastqc和cutadapt两个软件,Fastqc 使用conda 就可以安装。

    3. cutadapt下载源代码:https://github.com/marcelm/cutadapt

    安装:

              git clone https://github.com/marcelm/cutadapt/

              cd cutadapt

              python setup.py build_ext -i

    需要下载cython,下载安装还是无法进行

    没跑通,后面再看吧

    4.使用conda安装cutadapt

    cutadapt安装

    5.安装trim_galore

    export PATH=path$PATH

    已安装

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