- RunPCA时遇到error in irlba(A = t(x
- RunPCA时遇到error in irlba(A = t(x
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> pbmc <- RunPCA(pbmc, features = VariableFeatures(object = pbmc))
Error in irlba(A = t(x = object), nv = npcs, ...) : max(nu, nv) must be strictly less than min(nrow(A), ncol(A))
简单来说就是你的细胞数小于50。
npcs的选择是默认为50。PC当然要小于矩阵的行数或列数中的最小值。所以只需修改默认值即可。
解决方案：
pbmc <- RunPCA(pbmc, npcs = 33(填“细胞数-1”）,features = VariableFeatures(object = pbmc))
作者：木舟笔记
链接：https://www.jianshu.com/p/7e212d17d0ca
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