fastq文件长啥样

作者: Seurat_Satija | 来源:发表于2021-10-14 17:07 被阅读0次

fastq文件长啥样
记录一次单细胞分析流程
perl 命令行实战2 - fastq文件的相关操作
FastQC
sra转fastq
fastq 文件介绍
Hi-C分析练习（从cool文件到可视化热图）
FASTQ格式说明
RNA-seq分析（STAR-RSEM-DESeq2）
fastq-dump并行版pfastq-dump的使用

@ST-E00294:246:HHL7GALXX:7:1101:2402:2381 1:N:0:ATAGCG
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG
+
AAF-AFJFFJ-FFJAAFJJJJAJJJJJJFFJJ7FJJJJJJJJJJFJJ-FJJ@ST-E00294:246:HHL7GALXX:7:1101:3194:2381 1:N:0:ATAGCG
CCACTGCTAGCCTCTTTCCCTTTGCCCTCGCGGGGCTACTTTTTCCCCTCGCTCGCAGCCCTCTCCAAGACCGCGCAAGACACTGCCACGGCCCCGGGGCCGGGACGCCCTCCCAACCACAGCTCCTCCCGCCCGCCCAGCCGTCCTTCT
+
AA<-AA@ST-E00294:246:HHL7GALXX:7:1101:3356:2381 1:N:0:ATAGCG
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTG
+
AAA-F7F-AFJFJJJFJFFFFFJ<--<-F@ST-E00294:246:HHL7GALXX:7:1101:3599:2381 1:N:0:ATAGCG
GGCTTGGCCCCCGAGGCTGGACACAGACAGGTGTCCCCTGACATCTCCCGCGGCAGGTAGATCACCACACGGCAGGAGGTAGGCTTAGAAGGTTCTGCCGTCGTGGAAGTGATCACGAGTGAGGGGCCCTCAGAGGCCAGGGCCTCATAC
+
AAA-FJJFF-A-AFFAFJJFF7FAJ@ST-E00294:246:HHL7GALXX:7:1101:4229:2381 1:N:0:ATAGCG
GTTTTAAAGAGAAAAATGTTATGGATTACTACTTAGTGTGCATGCCTGGAGGGGAAGATAAAATGATGCTAATTTCCTCCCAGGAGTTTGGGAAATCATATTTCCTTCTATCCTGACCATCTTTACCTCTGCCCTAGCTAGGGAGTGAAT
+
@ST-E00294:246:HHL7GALXX:7:1101:4269:2381 1:N:0:ATAGCG
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGGCCCCCGCCACGCACCCCCGACCCCGAGAGACGCGCACCCGCGGCCGCTCCCGTCCCGTCCCGCTCCGACTGC
+
AAFFFJAFJAJJJJJAFJJJFJAFJJJJJJJJFJJ@ST-E00294:246:HHL7GALXX:7:1101:4310:2381 1:N:0:ATAGCG
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGAGACCGGAAGAGCAC
+
AA@ST-E00294:246:HHL7GALXX:7:1101:5467:2381 1:N:0:ATAGCG
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCCCCGGACGTGGGAGAGGGCGGCGGCGCCTCGTCCAGCCGCGGCGCGCGCCCAGCCCCGCTT
+
AAFAAJJJJ@ST-E00294:246:HHL7GALXX:7:1101:5751:2381 1:N:0:ATAGCG
CTTCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG
+
AAA@ST-E00294:246:HHL7GALXX:7:1101:6522:2381 1:N:0:ATAGCG
CGCGCCTGCTGCCTTCCTTGGATGCGGTAGCCGTTTCTCAGGCCCCCTCCCCGGAATCGAACCCTGATTCCCCGTCACCCGCGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAGATCGGAAGAGC
+
AAA-FJAFJFJJJAFJJJJ<