splineTimeR

Also known as splineTC

https://rdrr.io/bioc/splineTimeR/

https://github.com/ZytoHMGU/splineTimeR/

https://rdrr.io/bioc/splineTimeR/f/inst/doc/splineTimeR.pdf

https://bioconductor.org/packages/release/bioc/html/splineTimeR.html

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0160791

官方教程：

https://rdrr.io/bioc/splineTimeR/f/inst/doc/splineTimeR.pdf

1. ExpressionSet 数据结构

ExpressionSet 对象 是 splineTimeR 接受的输入数据的格式

https://www.jianshu.com/p/2ed89abbe9aa

http://www.bio-info-trainee.com/1510.html

构建 ExpressionSet:

https://jiangjun.link/post/bioconductor-expressionset/

使用 Biobase 包

#metadata_used: metadata
#matrix_used: expression matrix
phenoData_used <- new("AnnotatedDataFrame", data=metadata_used)#construct metadata object
ExpressionSet_object <- ExpressionSet(assayData=matrix_used, phenoData=phenoData_used)

2. 使用

GitHub: https://github.com/Yin-Zhiyong/R-Scripts/blob/main/splineTimeR/Create_ExpressionSet_object.R