利用二代测序数据矫正三代Nanopore测序数据

论文

https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02244-4

Ratatosk: hybrid error correction of long reads enables accurate variant calling and assembly

https://github.com/DecodeGenetics/Ratatosk

安装

git clone --recursive https://github.com/DecodeGenetics/Ratatosk.git
cd Ratatosk
mkdir build
cd bulid
cmake -DCMAKE_INSTALL_PREFIX=$(pwd) ..
make
make install

运行命令

~/scratch/apps/mingyan/Biotools/Ratatosk/build/bin/Ratatosk correct -v -c 16 -s ERR1878196_1.fastq.gz ERR1878196_2.fastq.gz -l ERR3284704.fastq.gz -o out_long_reads