TCID50计算器-python-pyqt5-可视化编程
运行环境
pyqt 5.13
python 3.7
Pycharm 2020.1 (安装插件 Qt Designer 和 Pyuic)
编程思路
先设计可视化ui代码
设计业务逻辑代码
设计主程序代码
在Pycharm中打开Qt Designer，手动设计好ui界面，保存为.ui文件
Qt Designer
在Pycharm中打开Qt Designer，利用Pyuic插件将ui_tcid50_4.ui文件生成相应ui_tcid50_4.py的文件，利于主程序调用
代码如下，末尾添加了测试代码
# -*- coding: utf-8 -*-
# Form implementation generated from reading ui file 'ui_tcid50_4.ui'
#
# Created by: PyQt5 UI code generator 5.13.0
#
# WARNING! All changes made in this file will be lost!
from PyQt5 import QtCore, QtGui, QtWidgets
class Ui_MainWindow(object):
def setupUi(self, MainWindow):
MainWindow.setObjectName("MainWindow")
MainWindow.resize(1121, 844)
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(12)
MainWindow.setFont(font)
self.centralwidget = QtWidgets.QWidget(MainWindow)
self.centralwidget.setObjectName("centralwidget")
self.groupBox = QtWidgets.QGroupBox(self.centralwidget)
self.groupBox.setGeometry(QtCore.QRect(30, 10, 1041, 411))
self.groupBox.setAutoFillBackground(False)
self.groupBox.setObjectName("groupBox")
self.lineEdit_10 = QtWidgets.QLineEdit(self.groupBox)
self.lineEdit_10.setGeometry(QtCore.QRect(340, 280, 51, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_10.setFont(font)
self.lineEdit_10.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_10.setObjectName("lineEdit_10")
self.label_2 = QtWidgets.QLabel(self.groupBox)
self.label_2.setGeometry(QtCore.QRect(40, 180, 281, 51))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_2.setFont(font)
self.label_2.setObjectName("label_2")
self.comboBox = QtWidgets.QComboBox(self.groupBox)
self.comboBox.setGeometry(QtCore.QRect(340, 190, 81, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.comboBox.setFont(font)
self.comboBox.setObjectName("comboBox")
self.comboBox.addItem("")
self.comboBox.addItem("")
self.comboBox.addItem("")
self.label_4 = QtWidgets.QLabel(self.groupBox)
self.label_4.setGeometry(QtCore.QRect(40, 280, 251, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_4.setFont(font)
self.label_4.setObjectName("label_4")
self.label_6 = QtWidgets.QLabel(self.groupBox)
self.label_6.setGeometry(QtCore.QRect(40, 330, 341, 61))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(18)
self.label_6.setFont(font)
self.label_6.setObjectName("label_6")
self.lineEdit_9 = QtWidgets.QLineEdit(self.groupBox)
self.lineEdit_9.setGeometry(QtCore.QRect(340, 240, 71, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_9.setFont(font)
self.lineEdit_9.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_9.setObjectName("lineEdit_9")
self.label_7 = QtWidgets.QLabel(self.groupBox)
self.label_7.setGeometry(QtCore.QRect(480, 350, 71, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(16)
self.label_7.setFont(font)
self.label_7.setObjectName("label_7")
self.label_5 = QtWidgets.QLabel(self.groupBox)
self.label_5.setGeometry(QtCore.QRect(420, 280, 121, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_5.setFont(font)
self.label_5.setObjectName("label_5")
self.horizontalLayoutWidget = QtWidgets.QWidget(self.groupBox)
self.horizontalLayoutWidget.setGeometry(QtCore.QRect(40, 80, 971, 71))
self.horizontalLayoutWidget.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.horizontalLayoutWidget.setFont(font)
self.horizontalLayoutWidget.setObjectName("horizontalLayoutWidget")
self.horizontalLayout = QtWidgets.QHBoxLayout(self.horizontalLayoutWidget)
self.horizontalLayout.setContentsMargins(0, 0, 0, 0)
self.horizontalLayout.setObjectName("horizontalLayout")
self.lineEdit = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit.setFont(font)
self.lineEdit.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit.setObjectName("lineEdit")
self.horizontalLayout.addWidget(self.lineEdit)
spacerItem = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem)
self.lineEdit_2 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_2.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_2.setFont(font)
self.lineEdit_2.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_2.setObjectName("lineEdit_2")
self.horizontalLayout.addWidget(self.lineEdit_2)
spacerItem1 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem1)
self.lineEdit_3 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_3.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_3.setFont(font)
self.lineEdit_3.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_3.setObjectName("lineEdit_3")
self.horizontalLayout.addWidget(self.lineEdit_3)
spacerItem2 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem2)
self.lineEdit_4 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_4.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_4.setFont(font)
self.lineEdit_4.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_4.setObjectName("lineEdit_4")
self.horizontalLayout.addWidget(self.lineEdit_4)
spacerItem3 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem3)
self.lineEdit_5 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_5.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_5.setFont(font)
self.lineEdit_5.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_5.setObjectName("lineEdit_5")
self.horizontalLayout.addWidget(self.lineEdit_5)
spacerItem4 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem4)
self.lineEdit_6 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_6.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_6.setFont(font)
self.lineEdit_6.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_6.setObjectName("lineEdit_6")
self.horizontalLayout.addWidget(self.lineEdit_6)
spacerItem5 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem5)
self.lineEdit_7 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_7.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_7.setFont(font)
self.lineEdit_7.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_7.setObjectName("lineEdit_7")
self.horizontalLayout.addWidget(self.lineEdit_7)
spacerItem6 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem6)
self.lineEdit_8 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_8.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(18)
self.lineEdit_8.setFont(font)
self.lineEdit_8.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_8.setObjectName("lineEdit_8")
self.horizontalLayout.addWidget(self.lineEdit_8)
self.label = QtWidgets.QLabel(self.groupBox)
self.label.setGeometry(QtCore.QRect(40, 50, 571, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label.setFont(font)
self.label.setObjectName("label")
self.pushButton = QtWidgets.QPushButton(self.groupBox)
self.pushButton.setGeometry(QtCore.QRect(720, 300, 141, 81))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton.setFont(font)
self.pushButton.setObjectName("pushButton")
self.lineEdit_11 = QtWidgets.QLineEdit(self.groupBox)
self.lineEdit_11.setGeometry(QtCore.QRect(380, 320, 91, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_11.setFont(font)
self.lineEdit_11.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_11.setObjectName("lineEdit_11")
self.pushButton_2 = QtWidgets.QPushButton(self.groupBox)
self.pushButton_2.setGeometry(QtCore.QRect(860, 160, 111, 51))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_2.setFont(font)
self.pushButton_2.setObjectName("pushButton_2")
self.label_3 = QtWidgets.QLabel(self.groupBox)
self.label_3.setGeometry(QtCore.QRect(40, 240, 261, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_3.setFont(font)
self.label_3.setObjectName("label_3")
self.groupBox_2 = QtWidgets.QGroupBox(self.centralwidget)
self.groupBox_2.setGeometry(QtCore.QRect(30, 430, 911, 281))
self.groupBox_2.setObjectName("groupBox_2")
self.label_8 = QtWidgets.QLabel(self.groupBox_2)
self.label_8.setGeometry(QtCore.QRect(480, 60, 71, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(16)
self.label_8.setFont(font)
self.label_8.setText("")
self.label_8.setObjectName("label_8")
self.lineEdit_12 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_12.setGeometry(QtCore.QRect(340, 20, 91, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_12.setFont(font)
self.lineEdit_12.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_12.setObjectName("lineEdit_12")
self.label_9 = QtWidgets.QLabel(self.groupBox_2)
self.label_9.setGeometry(QtCore.QRect(40, 40, 311, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(18)
self.label_9.setFont(font)
self.label_9.setObjectName("label_9")
self.label_10 = QtWidgets.QLabel(self.groupBox_2)
self.label_10.setGeometry(QtCore.QRect(440, 50, 71, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(16)
self.label_10.setFont(font)
self.label_10.setObjectName("label_10")
self.label_12 = QtWidgets.QLabel(self.groupBox_2)
self.label_12.setGeometry(QtCore.QRect(40, 180, 131, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_12.setFont(font)
self.label_12.setObjectName("label_12")
self.label_13 = QtWidgets.QLabel(self.groupBox_2)
self.label_13.setGeometry(QtCore.QRect(270, 180, 371, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_13.setFont(font)
self.label_13.setObjectName("label_13")
self.lineEdit_13 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_13.setGeometry(QtCore.QRect(180, 170, 71, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_13.setFont(font)
self.lineEdit_13.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_13.setObjectName("lineEdit_13")
self.label_14 = QtWidgets.QLabel(self.groupBox_2)
self.label_14.setGeometry(QtCore.QRect(40, 120, 131, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_14.setFont(font)
self.label_14.setObjectName("label_14")
self.lineEdit_14 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_14.setGeometry(QtCore.QRect(160, 120, 51, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_14.setFont(font)
self.lineEdit_14.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_14.setObjectName("lineEdit_14")
self.lineEdit_15 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_15.setGeometry(QtCore.QRect(270, 100, 41, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_15.setFont(font)
self.lineEdit_15.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_15.setObjectName("lineEdit_15")
self.label_15 = QtWidgets.QLabel(self.groupBox_2)
self.label_15.setGeometry(QtCore.QRect(320, 130, 21, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_15.setFont(font)
self.label_15.setObjectName("label_15")
self.lineEdit_22 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_22.setGeometry(QtCore.QRect(520, 170, 111, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_22.setFont(font)
self.lineEdit_22.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_22.setObjectName("lineEdit_22")
self.label_22 = QtWidgets.QLabel(self.groupBox_2)
self.label_22.setGeometry(QtCore.QRect(640, 180, 21, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_22.setFont(font)
self.label_22.setObjectName("label_22")
self.label_23 = QtWidgets.QLabel(self.groupBox_2)
self.label_23.setGeometry(QtCore.QRect(220, 130, 51, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_23.setFont(font)
self.label_23.setObjectName("label_23")
self.pushButton_3 = QtWidgets.QPushButton(self.groupBox_2)
self.pushButton_3.setGeometry(QtCore.QRect(730, 170, 141, 71))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_3.setFont(font)
self.pushButton_3.setObjectName("pushButton_3")
self.label_24 = QtWidgets.QLabel(self.groupBox_2)
self.label_24.setGeometry(QtCore.QRect(40, 230, 241, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_24.setFont(font)
self.label_24.setObjectName("label_24")
self.lineEdit_16 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_16.setGeometry(QtCore.QRect(270, 220, 61, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_16.setFont(font)
self.lineEdit_16.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_16.setObjectName("lineEdit_16")
self.label_25 = QtWidgets.QLabel(self.groupBox_2)
self.label_25.setGeometry(QtCore.QRect(340, 230, 21, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_25.setFont(font)
self.label_25.setObjectName("label_25")
self.pushButton_4 = QtWidgets.QPushButton(self.groupBox_2)
self.pushButton_4.setGeometry(QtCore.QRect(690, 40, 91, 51))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_4.setFont(font)
self.pushButton_4.setObjectName("pushButton_4")
self.label_26 = QtWidgets.QLabel(self.groupBox_2)
self.label_26.setGeometry(QtCore.QRect(150, 200, 121, 21))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(10)
self.label_26.setFont(font)
self.label_26.setObjectName("label_26")
self.label_27 = QtWidgets.QLabel(self.groupBox_2)
self.label_27.setGeometry(QtCore.QRect(270, 250, 71, 21))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(10)
self.label_27.setFont(font)
self.label_27.setObjectName("label_27")
self.label_28 = QtWidgets.QLabel(self.groupBox_2)
self.label_28.setGeometry(QtCore.QRect(360, 130, 191, 21))
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(10)
self.label_28.setFont(font)
self.label_28.setObjectName("label_28")
self.pushButton_6 = QtWidgets.QPushButton(self.groupBox_2)
self.pushButton_6.setGeometry(QtCore.QRect(500, 30, 51, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(10)
font.setBold(True)
font.setWeight(75)
self.pushButton_6.setFont(font)
self.pushButton_6.setLayoutDirection(QtCore.Qt.LeftToRight)
self.pushButton_6.setAutoFillBackground(False)
self.pushButton_6.setAutoExclusive(False)
self.pushButton_6.setObjectName("pushButton_6")
self.pushButton_5 = QtWidgets.QPushButton(self.centralwidget)
self.pushButton_5.setGeometry(QtCore.QRect(1000, 530, 81, 141))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_5.setFont(font)
self.pushButton_5.setObjectName("pushButton_5")
self.pushButton_7 = QtWidgets.QPushButton(self.centralwidget)
self.pushButton_7.setGeometry(QtCore.QRect(620, 760, 81, 31))
self.pushButton_7.setObjectName("pushButton_7")
self.pushButton_8 = QtWidgets.QPushButton(self.centralwidget)
self.pushButton_8.setGeometry(QtCore.QRect(340, 760, 221, 31))
self.pushButton_8.setObjectName("pushButton_8")
self.label_11 = QtWidgets.QLabel(self.centralwidget)
self.label_11.setGeometry(QtCore.QRect(990, 769, 101, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(10)
self.label_11.setFont(font)
self.label_11.setAlignment(QtCore.Qt.AlignCenter)
self.label_11.setObjectName("label_11")
MainWindow.setCentralWidget(self.centralwidget)
self.menubar = QtWidgets.QMenuBar(MainWindow)
self.menubar.setGeometry(QtCore.QRect(0, 0, 1121, 23))
self.menubar.setObjectName("menubar")
MainWindow.setMenuBar(self.menubar)
self.statusbar = QtWidgets.QStatusBar(MainWindow)
self.statusbar.setObjectName("statusbar")
MainWindow.setStatusBar(self.statusbar)
self.retranslateUi(MainWindow)
self.pushButton_4.clicked.connect(self.lineEdit_12.clear)
self.pushButton_4.clicked.connect(self.lineEdit_13.clear)
self.pushButton_4.clicked.connect(self.lineEdit_22.clear)
self.pushButton_4.clicked.connect(self.lineEdit_16.clear)
self.pushButton_2.clicked.connect(self.lineEdit.clear)
self.pushButton_2.clicked.connect(self.lineEdit_2.clear)
self.pushButton_2.clicked.connect(self.lineEdit_3.clear)
self.pushButton_2.clicked.connect(self.lineEdit_4.clear)
self.pushButton_2.clicked.connect(self.lineEdit_5.clear)
self.pushButton_2.clicked.connect(self.lineEdit_6.clear)
self.pushButton_2.clicked.connect(self.lineEdit_7.clear)
self.pushButton_2.clicked.connect(self.lineEdit_8.clear)
self.pushButton_5.clicked.connect(MainWindow.close)
self.pushButton_6.clicked.connect(self.lineEdit_12.show)
self.pushButton.clicked.connect(self.lineEdit_11.show)
self.pushButton_3.clicked.connect(self.lineEdit_22.show)
self.pushButton_3.clicked.connect(self.lineEdit_16.show)
self.pushButton_3.clicked.connect(self.lineEdit_13.show)
self.pushButton_2.clicked.connect(self.lineEdit_11.clear)
QtCore.QMetaObject.connectSlotsByName(MainWindow)
def retranslateUi(self, MainWindow):
_translate = QtCore.QCoreApplication.translate
MainWindow.setWindowTitle(_translate("MainWindow", "MainWindow"))
self.groupBox.setTitle(_translate("MainWindow", "TCID50 计算"))
self.lineEdit_10.setText(_translate("MainWindow", "-1"))
self.label_2.setText(_translate("MainWindow", "稀释梯度为10倍，每孔上液量为"))
self.comboBox.setItemText(0, _translate("MainWindow", "100uL"))
self.comboBox.setItemText(1, _translate("MainWindow", "200uL"))
self.comboBox.setItemText(2, _translate("MainWindow", "1mL"))
self.label_4.setText(_translate("MainWindow", "最低稀释倍数的lg负对数为:"))
self.label_6.setText(_translate("MainWindow", "待检原液的TCID50的值为： 10"))
self.lineEdit_9.setText(_translate("MainWindow", "8"))
self.label_7.setText(_translate("MainWindow", "/mL"))
self.label_5.setText(_translate("MainWindow", "(如-2， -4)"))
self.lineEdit.setText(_translate("MainWindow", "8"))
self.lineEdit_2.setText(_translate("MainWindow", "8"))
self.lineEdit_3.setText(_translate("MainWindow", "7"))
self.lineEdit_4.setText(_translate("MainWindow", "3"))
self.lineEdit_5.setText(_translate("MainWindow", "1"))
self.lineEdit_6.setText(_translate("MainWindow", "0"))
self.lineEdit_7.setText(_translate("MainWindow", "0"))
self.lineEdit_8.setText(_translate("MainWindow", "0"))
self.label.setText(_translate("MainWindow", "请填写各稀释度的病变孔数（从低稀释倍数开始填）（自然数）"))
self.pushButton.setText(_translate("MainWindow", "计算 TCID50"))
self.pushButton_2.setText(_translate("MainWindow", "清除数据"))
self.label_3.setText(_translate("MainWindow", "每个稀释度重复数为:"))
self.groupBox_2.setTitle(_translate("MainWindow", "MOI 计算"))
self.lineEdit_12.setText(_translate("MainWindow", "-6"))
self.label_9.setText(_translate("MainWindow", "原液的TCID50的值为： 10"))
self.label_10.setText(_translate("MainWindow", "/mL"))
self.label_12.setText(_translate("MainWindow", "要计算MOI为"))
self.label_13.setText(_translate("MainWindow", "时，需要加入的病毒液的量为"))
self.lineEdit_13.setText(_translate("MainWindow", "1"))
self.label_14.setText(_translate("MainWindow", "细胞数量为："))
self.lineEdit_14.setText(_translate("MainWindow", "2"))
self.lineEdit_15.setText(_translate("MainWindow", "6"))
self.label_15.setText(_translate("MainWindow", "个"))
self.lineEdit_22.setText(_translate("MainWindow", "100"))
self.label_22.setText(_translate("MainWindow", "uL"))
self.label_23.setText(_translate("MainWindow", "X 10"))
self.pushButton_3.setText(_translate("MainWindow", "计算 MOI"))
self.label_24.setText(_translate("MainWindow", "此时，感染的细胞比例为："))
self.lineEdit_16.setText(_translate("MainWindow", "90"))
self.label_25.setText(_translate("MainWindow", "%"))
self.pushButton_4.setText(_translate("MainWindow", "清除"))
self.label_26.setText(_translate("MainWindow", "（PFU / cell_count）"))
self.label_27.setText(_translate("MainWindow", "（0-99.99）"))
self.label_28.setText(_translate("MainWindow", "(六孔板一般为：2x10^6个细胞)"))
self.pushButton_6.setText(_translate("MainWindow", "填充"))
self.pushButton_5.setText(_translate("MainWindow", "退出"))
self.pushButton_7.setText(_translate("MainWindow", "帮助"))
self.pushButton_8.setText(_translate("MainWindow", "Copyright © 2020 SCAU"))
self.label_11.setText(_translate("MainWindow", "版本号：4.0.1"))
# 此处为额外添加的测试代码
if __name__ == "__main__":
ui = Ui_MainWindow()
ui.setupUi()
业务代码
设计业务代码，生成有逻辑业务的界面新建 myTCID50.py,调用导入ui_tcid50_4.py文件中Ui_MainWindow类
业务内容：根据输入的原始数据及参数，调用函数方法计算结果并显示在相应位置
本TCID50计算器采用Reed-Muench法计算病毒的滴度
代码如下：
##与UI窗体类对应的业务逻辑类
import math
import sys
from PyQt5.QtWidgets import QApplication, QMainWindow
from PyQt5.QtCore import Qt, pyqtSlot
from ui_tcid50_4 import Ui_MainWindow
import PyQt5.QtWidgets as qw
def jisuan(a, repeatNum):
# a = [] # 各CPE孔数
b = [] # 各非CPE孔数
a_acc = [] # 各CPE累积孔数
b_acc = [] # 各非CPE累积孔数
ab_acc = [] # 各孔累积和
a_ratio = [] # CPE累积孔数占比
# 将列表 字符串类型转换成整型
a = list(map(int, a))
# 显示输入的CPE孔数
print("\n CPE孔数为：\n ", a)
# 计算 个稀释度非CPE孔数
for i in range(0, 8, 1):
b.append(repeatNum - a[i])
print("\n 非CPE孔素为：\n ", b)
# 计算CPE累积孔数，从高稀释倍数到低稀释倍数
s = 0
for i in reversed(a):
s = s + i
a_acc.append(s)
a_acc.reverse()
print("\n CPE累积为：\n ", a_acc)
#计算非CPE累积孔数
s = 0
for i in b:
s = s + i
b_acc.append(s)
print("\n 非CPE累积为：\n ", b_acc)
# 计算总累积孔数
for m, n in zip(a_acc, b_acc):
ab_acc.append(m + n)
print("\n 孔累积和为：\n ", ab_acc)
# 计算各CPE累积孔数 占 总累积孔数（CPE累积和非CPE累积）的 比例
for m, n in zip(a_acc, ab_acc):
a_ratio.append(m / n)
for i, n in zip(range(8), a_ratio):
a_ratio[i] = '{0:.4f}'.format(a_ratio[i])
print("\n CPE累积占比为：\n ", a_ratio)
# 寻找大于等于最接近于50%的稀释度
# 寻找大于等于最接近于50%的稀释度 占率
gd50 = 0
for i, i2 in zip(reversed(a_ratio), range(8)):
if round(float(i)) >= 0.5:
gd50z = float(i)
gd50 = 8 - i2 - 1
break
# 寻找小于50%且最近于50%的稀释度 占率
for i in a_ratio:
if round(float(i)) < 0.5:
d50 = i
break
# 计算距离
s = (float(gd50z) - 0.5) / (float(gd50z) - float(d50))
print("\n 高于50%的稀释度为：{} 其比率为：{} ".format(gd50, gd50z))
# 计算lg(TCID50) =
tcid50 = -(gd50 + 1 + s)
print(tcid50)
# tcid50 = format(tcid50, '.4f')
return tcid50
def test_virus_volume(tcid50_value_init, volume_index):
if volume_index == 0:
tcid50 = tcid50_value_init - 1
elif volume_index == 1:
tcid50 = tcid50_value_init - math.log(5, 10)
else:
tcid50 = tcid50_value_init
return tcid50
def test_init_dilution_factor(tcid50_value, initFactor):
tcid50 = tcid50_value + 1 + initFactor
return tcid50
def virus_volume_need(tcid_fuzhi, cell_xi, cell_zhi, moi_value):
cell_count = cell_xi * (10 ** cell_zhi)
denominator = 0.7 * (10 ** (-tcid_fuzhi))
virus_volume = ((moi_value * cell_count) / denominator) * 1000 # 变微升
virus_volume = format(virus_volume, '.2f')
print(tcid_fuzhi)
# print(cell_xi)
# print(cell_zhi)
# print(moi_value)
# print(cell_count)
# print(denominator)
# print(virus_volume)
return str(virus_volume)
def infect_rate(moi_value):
p0 = math.e ** (-moi_value)
p1 = (1 - p0) * 100
p1 = format(p1, '.2f')
m = -math.log(p0, math.e)
print("p0", p0)
print("moi", m)
return str(p1)
class QmyDialog(QMainWindow):
def __init__(self, parent=None):
super().__init__(parent) # 调用父类构造函数，创建窗体
self.ui = Ui_MainWindow() # 创建UI对象
self.ui.setupUi(self) # 构造UI界面
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_clicked(self):
a1 = a2 = a3 = a4 = a5 = a6 = a7 = a8 = 0
a = [a1, a2, a3, a4, a5, a6, a7, a8]
if self.ui.lineEdit.text() == "":
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'提示')
msgBox.setText(u"\n警告!\n请输入正确的数据\n请确认是否输入数据！")
# qw.QMessageBox.about(self, "警告", "请输入正确的数据！")
msgBox.exec_() # 模态对话框
else:
try:
a1 = float(self.ui.lineEdit.text())
a2 = float(self.ui.lineEdit_2.text())
a3 = float(self.ui.lineEdit_3.text())
a4 = float(self.ui.lineEdit_4.text())
a5 = float(self.ui.lineEdit_5.text())
a6 = float(self.ui.lineEdit_6.text())
a7 = float(self.ui.lineEdit_7.text())
a8 = float(self.ui.lineEdit_8.text())
except:
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'提示')
msgBox.setText(u"\n警告!\n请输入正确的数据\n请确认输入数据是否完整！")
msgBox.exec_() # 模态对话框
else:
# a_sum = a1 + a2 + a3 + a4 + a5 + a6 + a7 + a8
# 输入数据无异常，进行计算初试TCID50
a = [a1, a2, a3, a4, a5, a6, a7, a8]
repeatNum = int(self.ui.lineEdit_9.text())
print(repeatNum)
tcid50_value_init = float(jisuan(a, repeatNum))
# 考虑毒液测试体积，通常为100uL，index为0
volume_index = self.ui.comboBox.currentIndex()
tcid50 = test_virus_volume(tcid50_value_init, volume_index)
# 考虑起始稀释倍数,负对数通常为-1
initFactor = int(self.ui.lineEdit_10.text())
tcid50 = test_init_dilution_factor(tcid50, initFactor)
# 格式化输出
tcid50 = format(tcid50, '.4f')
self.ui.lineEdit_11.setText(str(tcid50))
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_3_clicked(self):
try:
tcid_fuzhi = float(self.ui.lineEdit_12.text()) # TCID50
cell_xi = float(self.ui.lineEdit_14.text()) # 细胞数量 系数
cell_zhi = float(self.ui.lineEdit_15.text()) # 细胞数量 指数
moi_value = float(self.ui.lineEdit_13.text()) # MOI值
except:
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'提示')
msgBox.setText(u"\n警告!\n请输入正确的数据\n请确认输入数据是否完整！\n")
# qw.QMessageBox.about(self, "警告", "请输入正确的数据！")
msgBox.exec_() # 模态对话框
else:
virus_volume_uL = virus_volume_need(tcid_fuzhi, cell_xi, cell_zhi, moi_value)
self.ui.lineEdit_22.setText(virus_volume_uL)
infect_rate100 = infect_rate(moi_value)
self.ui.lineEdit_16.setText(infect_rate100)
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_6_clicked(self):
tcid_last = self.ui.lineEdit_11.text()
self.ui.lineEdit_12.setText(tcid_last)
if __name__ == "__main__": # 用于当前窗体测试
app = QApplication(sys.argv) # 创建GUI应用程序
form = QmyDialog() # 创建窗体
form.show()
sys.exit(app.exec_())
os.system(pause)
主调用程序
设计主调用程序，方便简洁
新建appMain_TICD50_4.py,导入myTCID50.py中QmyDialog类
主调用程序代码如下：
#!/usr/bin/env python
# -- coding = 'utf-8' --
# @Python : 3.7
# @OS : Windows 10 kiton.
# @Time : 2021/3/9 9:26
# @Author : Ai Qiangyun
# @E-mail : 1154282938@qq.com
# @File : appMain_TCID50_4.py.py
# @Software: PyCharm
## GUI应用程序主程序入口
import sys
from PyQt5.QtWidgets import QApplication
from myTCID50_4 import QmyDialog
app = QApplication(sys.argv) # 创建GUI应用程序
mainform = QmyDialog() # 创建主窗体
mainform.show() # 显示主窗体
sys.exit(app.exec_())
在Pycharm 中点击运行主程序，或者在终端运行程序，代码如下：
python appMain_TICD50_4.py
结果如下：
TCID50_4计算器界面
输入数据 8 8 8 7 3 1 0 0，点击计算按钮，生成结果，10^(-4.8065)
TCID50_4计算器界面
总结
TCID50计算器走过了几个年头
第二版 2.0 (2017.7.19 VB6.0开发）
TCID50计算器 2.0
2.0 帮助信息
第三版 3.0.1（2018.3.31 VB6.0开发）
TCID50计算器 3.0
3.0.1 帮助信息
第四版 （4.0.0为python 控制台，没有可视化界面）
第四版 4.0.1（此版）为python + pyqt可视化界面版，另外添加了MOI计算，属于用户友好型
TCID50计算器 4.0.1
第四版 4.0.2 （最终版,2020.11.26） 完善，添加了帮助信息和版权信息
TCID50计算器 4.0.2
4.0.2 帮助
最终版为4.0.2，所有代码写在一个文件TCID50-4-2.py
代码如下：
# -*- coding: utf-8 -*-
# Form implementation generated from reading ui file 'ui_tcid50_4.ui'
#
# Created by: PyQt5 UI code generator 5.13.0
#
# WARNING! All changes made in this file will be lost!
from PyQt5 import QtCore, QtGui, QtWidgets
class Ui_MainWindow(object):
def setupUi(self, MainWindow):
MainWindow.setObjectName("MainWindow")
MainWindow.resize(1331, 858)
font = QtGui.QFont()
font.setFamily("Arial")
font.setPointSize(12)
MainWindow.setFont(font)
self.centralwidget = QtWidgets.QWidget(MainWindow)
self.centralwidget.setObjectName("centralwidget")
self.groupBox = QtWidgets.QGroupBox(self.centralwidget)
self.groupBox.setGeometry(QtCore.QRect(30, 10, 1041, 391))
font = QtGui.QFont()
font.setPointSize(14)
self.groupBox.setFont(font)
self.groupBox.setAutoFillBackground(False)
self.groupBox.setObjectName("groupBox")
self.lineEdit_10 = QtWidgets.QLineEdit(self.groupBox)
self.lineEdit_10.setGeometry(QtCore.QRect(380, 280, 51, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_10.setFont(font)
self.lineEdit_10.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_10.setObjectName("lineEdit_10")
self.label_2 = QtWidgets.QLabel(self.groupBox)
self.label_2.setGeometry(QtCore.QRect(40, 180, 311, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_2.setFont(font)
self.label_2.setObjectName("label_2")
self.comboBox = QtWidgets.QComboBox(self.groupBox)
self.comboBox.setGeometry(QtCore.QRect(380, 180, 81, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.comboBox.setFont(font)
self.comboBox.setObjectName("comboBox")
self.comboBox.addItem("")
self.comboBox.addItem("")
self.comboBox.addItem("")
self.label_4 = QtWidgets.QLabel(self.groupBox)
self.label_4.setGeometry(QtCore.QRect(40, 280, 291, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_4.setFont(font)
self.label_4.setObjectName("label_4")
self.label_6 = QtWidgets.QLabel(self.groupBox)
self.label_6.setGeometry(QtCore.QRect(40, 330, 361, 61))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_6.setFont(font)
self.label_6.setObjectName("label_6")
self.lineEdit_9 = QtWidgets.QLineEdit(self.groupBox)
self.lineEdit_9.setGeometry(QtCore.QRect(380, 230, 71, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_9.setFont(font)
self.lineEdit_9.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_9.setObjectName("lineEdit_9")
self.label_7 = QtWidgets.QLabel(self.groupBox)
self.label_7.setGeometry(QtCore.QRect(480, 350, 71, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_7.setFont(font)
self.label_7.setObjectName("label_7")
self.label_5 = QtWidgets.QLabel(self.groupBox)
self.label_5.setGeometry(QtCore.QRect(490, 280, 121, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_5.setFont(font)
self.label_5.setObjectName("label_5")
self.horizontalLayoutWidget = QtWidgets.QWidget(self.groupBox)
self.horizontalLayoutWidget.setGeometry(QtCore.QRect(40, 80, 988, 71))
self.horizontalLayoutWidget.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.horizontalLayoutWidget.setFont(font)
self.horizontalLayoutWidget.setObjectName("horizontalLayoutWidget")
self.horizontalLayout = QtWidgets.QHBoxLayout(self.horizontalLayoutWidget)
self.horizontalLayout.setContentsMargins(0, 0, 0, 0)
self.horizontalLayout.setObjectName("horizontalLayout")
self.lineEdit = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit.setFont(font)
self.lineEdit.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit.setObjectName("lineEdit")
self.horizontalLayout.addWidget(self.lineEdit)
spacerItem = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem)
self.lineEdit_2 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_2.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_2.setFont(font)
self.lineEdit_2.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_2.setObjectName("lineEdit_2")
self.horizontalLayout.addWidget(self.lineEdit_2)
spacerItem1 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem1)
self.lineEdit_3 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_3.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_3.setFont(font)
self.lineEdit_3.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_3.setObjectName("lineEdit_3")
self.horizontalLayout.addWidget(self.lineEdit_3)
spacerItem2 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem2)
self.lineEdit_4 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_4.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_4.setFont(font)
self.lineEdit_4.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_4.setObjectName("lineEdit_4")
self.horizontalLayout.addWidget(self.lineEdit_4)
spacerItem3 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem3)
self.lineEdit_5 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_5.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_5.setFont(font)
self.lineEdit_5.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_5.setObjectName("lineEdit_5")
self.horizontalLayout.addWidget(self.lineEdit_5)
spacerItem4 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem4)
self.lineEdit_6 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_6.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_6.setFont(font)
self.lineEdit_6.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_6.setObjectName("lineEdit_6")
self.horizontalLayout.addWidget(self.lineEdit_6)
spacerItem5 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem5)
self.lineEdit_7 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_7.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_7.setFont(font)
self.lineEdit_7.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_7.setObjectName("lineEdit_7")
self.horizontalLayout.addWidget(self.lineEdit_7)
spacerItem6 = QtWidgets.QSpacerItem(60, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum)
self.horizontalLayout.addItem(spacerItem6)
self.lineEdit_8 = QtWidgets.QLineEdit(self.horizontalLayoutWidget)
self.lineEdit_8.setMinimumSize(QtCore.QSize(0, 40))
font = QtGui.QFont()
font.setPointSize(14)
self.lineEdit_8.setFont(font)
self.lineEdit_8.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_8.setObjectName("lineEdit_8")
self.horizontalLayout.addWidget(self.lineEdit_8)
self.label = QtWidgets.QLabel(self.groupBox)
self.label.setGeometry(QtCore.QRect(40, 40, 681, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label.setFont(font)
self.label.setObjectName("label")
self.pushButton = QtWidgets.QPushButton(self.groupBox)
self.pushButton.setGeometry(QtCore.QRect(750, 290, 141, 81))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
font.setBold(True)
font.setWeight(75)
self.pushButton.setFont(font)
self.pushButton.setObjectName("pushButton")
self.lineEdit_11 = QtWidgets.QLineEdit(self.groupBox)
self.lineEdit_11.setGeometry(QtCore.QRect(360, 330, 91, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_11.setFont(font)
self.lineEdit_11.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_11.setObjectName("lineEdit_11")
self.pushButton_2 = QtWidgets.QPushButton(self.groupBox)
self.pushButton_2.setGeometry(QtCore.QRect(860, 160, 111, 51))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
font.setBold(True)
font.setWeight(75)
self.pushButton_2.setFont(font)
self.pushButton_2.setObjectName("pushButton_2")
self.label_3 = QtWidgets.QLabel(self.groupBox)
self.label_3.setGeometry(QtCore.QRect(40, 230, 271, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_3.setFont(font)
self.label_3.setObjectName("label_3")
self.groupBox_2 = QtWidgets.QGroupBox(self.centralwidget)
self.groupBox_2.setGeometry(QtCore.QRect(40, 430, 951, 301))
font = QtGui.QFont()
font.setPointSize(12)
self.groupBox_2.setFont(font)
self.groupBox_2.setObjectName("groupBox_2")
self.label_8 = QtWidgets.QLabel(self.groupBox_2)
self.label_8.setGeometry(QtCore.QRect(480, 60, 71, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(16)
self.label_8.setFont(font)
self.label_8.setText("")
self.label_8.setObjectName("label_8")
self.lineEdit_12 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_12.setGeometry(QtCore.QRect(310, 20, 91, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_12.setFont(font)
self.lineEdit_12.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_12.setObjectName("lineEdit_12")
self.label_9 = QtWidgets.QLabel(self.groupBox_2)
self.label_9.setGeometry(QtCore.QRect(20, 40, 311, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_9.setFont(font)
self.label_9.setObjectName("label_9")
self.label_10 = QtWidgets.QLabel(self.groupBox_2)
self.label_10.setGeometry(QtCore.QRect(440, 40, 71, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_10.setFont(font)
self.label_10.setObjectName("label_10")
self.label_12 = QtWidgets.QLabel(self.groupBox_2)
self.label_12.setGeometry(QtCore.QRect(20, 190, 151, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_12.setFont(font)
self.label_12.setObjectName("label_12")
self.label_13 = QtWidgets.QLabel(self.groupBox_2)
self.label_13.setGeometry(QtCore.QRect(270, 190, 301, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_13.setFont(font)
self.label_13.setObjectName("label_13")
self.lineEdit_13 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_13.setGeometry(QtCore.QRect(180, 180, 71, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_13.setFont(font)
self.lineEdit_13.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_13.setObjectName("lineEdit_13")
self.label_14 = QtWidgets.QLabel(self.groupBox_2)
self.label_14.setGeometry(QtCore.QRect(20, 120, 131, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_14.setFont(font)
self.label_14.setObjectName("label_14")
self.lineEdit_14 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_14.setGeometry(QtCore.QRect(160, 120, 91, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_14.setFont(font)
self.lineEdit_14.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_14.setObjectName("lineEdit_14")
self.lineEdit_15 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_15.setGeometry(QtCore.QRect(320, 100, 51, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_15.setFont(font)
self.lineEdit_15.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_15.setObjectName("lineEdit_15")
self.label_15 = QtWidgets.QLabel(self.groupBox_2)
self.label_15.setGeometry(QtCore.QRect(400, 130, 21, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_15.setFont(font)
self.label_15.setObjectName("label_15")
self.lineEdit_22 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_22.setGeometry(QtCore.QRect(580, 180, 111, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_22.setFont(font)
self.lineEdit_22.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_22.setObjectName("lineEdit_22")
self.label_22 = QtWidgets.QLabel(self.groupBox_2)
self.label_22.setGeometry(QtCore.QRect(700, 190, 21, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_22.setFont(font)
self.label_22.setObjectName("label_22")
self.label_23 = QtWidgets.QLabel(self.groupBox_2)
self.label_23.setGeometry(QtCore.QRect(260, 130, 51, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_23.setFont(font)
self.label_23.setObjectName("label_23")
self.pushButton_3 = QtWidgets.QPushButton(self.groupBox_2)
self.pushButton_3.setGeometry(QtCore.QRect(760, 220, 141, 71))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_3.setFont(font)
self.pushButton_3.setObjectName("pushButton_3")
self.label_24 = QtWidgets.QLabel(self.groupBox_2)
self.label_24.setGeometry(QtCore.QRect(20, 250, 311, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(12)
self.label_24.setFont(font)
self.label_24.setObjectName("label_24")
self.lineEdit_16 = QtWidgets.QLineEdit(self.groupBox_2)
self.lineEdit_16.setGeometry(QtCore.QRect(300, 240, 91, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.lineEdit_16.setFont(font)
self.lineEdit_16.setAlignment(QtCore.Qt.AlignCenter)
self.lineEdit_16.setObjectName("lineEdit_16")
self.label_25 = QtWidgets.QLabel(self.groupBox_2)
self.label_25.setGeometry(QtCore.QRect(400, 250, 21, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
self.label_25.setFont(font)
self.label_25.setObjectName("label_25")
self.pushButton_4 = QtWidgets.QPushButton(self.groupBox_2)
self.pushButton_4.setGeometry(QtCore.QRect(690, 40, 91, 51))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_4.setFont(font)
self.pushButton_4.setObjectName("pushButton_4")
self.pushButton_6 = QtWidgets.QPushButton(self.groupBox_2)
self.pushButton_6.setGeometry(QtCore.QRect(540, 30, 51, 31))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(10)
font.setBold(True)
font.setWeight(75)
self.pushButton_6.setFont(font)
self.pushButton_6.setLayoutDirection(QtCore.Qt.LeftToRight)
self.pushButton_6.setAutoFillBackground(False)
self.pushButton_6.setAutoExclusive(False)
self.pushButton_6.setObjectName("pushButton_6")
self.pushButton_5 = QtWidgets.QPushButton(self.centralwidget)
self.pushButton_5.setGeometry(QtCore.QRect(1030, 530, 81, 141))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(14)
font.setBold(True)
font.setWeight(75)
self.pushButton_5.setFont(font)
self.pushButton_5.setObjectName("pushButton_5")
self.pushButton_7 = QtWidgets.QPushButton(self.centralwidget)
self.pushButton_7.setGeometry(QtCore.QRect(620, 760, 81, 31))
self.pushButton_7.setObjectName("pushButton_7")
self.pushButton_8 = QtWidgets.QPushButton(self.centralwidget)
self.pushButton_8.setGeometry(QtCore.QRect(340, 760, 221, 31))
self.pushButton_8.setObjectName("pushButton_8")
self.label_11 = QtWidgets.QLabel(self.centralwidget)
self.label_11.setGeometry(QtCore.QRect(990, 769, 101, 21))
font = QtGui.QFont()
font.setFamily("等线")
font.setPointSize(10)
self.label_11.setFont(font)
self.label_11.setAlignment(QtCore.Qt.AlignCenter)
self.label_11.setObjectName("label_11")
MainWindow.setCentralWidget(self.centralwidget)
self.menubar = QtWidgets.QMenuBar(MainWindow)
self.menubar.setGeometry(QtCore.QRect(0, 0, 1331, 26))
self.menubar.setObjectName("menubar")
MainWindow.setMenuBar(self.menubar)
self.statusbar = QtWidgets.QStatusBar(MainWindow)
self.statusbar.setObjectName("statusbar")
MainWindow.setStatusBar(self.statusbar)
self.retranslateUi(MainWindow)
self.pushButton_4.clicked.connect(self.lineEdit_12.clear)
self.pushButton_4.clicked.connect(self.lineEdit_13.clear)
self.pushButton_4.clicked.connect(self.lineEdit_22.clear)
self.pushButton_4.clicked.connect(self.lineEdit_16.clear)
self.pushButton_2.clicked.connect(self.lineEdit.clear)
self.pushButton_2.clicked.connect(self.lineEdit_2.clear)
self.pushButton_2.clicked.connect(self.lineEdit_3.clear)
self.pushButton_2.clicked.connect(self.lineEdit_4.clear)
self.pushButton_2.clicked.connect(self.lineEdit_5.clear)
self.pushButton_2.clicked.connect(self.lineEdit_6.clear)
self.pushButton_2.clicked.connect(self.lineEdit_7.clear)
self.pushButton_2.clicked.connect(self.lineEdit_8.clear)
self.pushButton_5.clicked.connect(MainWindow.close)
self.pushButton_6.clicked.connect(self.lineEdit_12.show)
self.pushButton.clicked.connect(self.lineEdit_11.show)
self.pushButton_3.clicked.connect(self.lineEdit_22.show)
self.pushButton_3.clicked.connect(self.lineEdit_16.show)
self.pushButton_3.clicked.connect(self.lineEdit_13.show)
self.pushButton_2.clicked.connect(self.lineEdit_11.clear)
QtCore.QMetaObject.connectSlotsByName(MainWindow)
def retranslateUi(self, MainWindow):
_translate = QtCore.QCoreApplication.translate
MainWindow.setWindowTitle(_translate("MainWindow", "MainWindow"))
self.groupBox.setTitle(_translate("MainWindow", "TCID50 计算"))
self.lineEdit_10.setText(_translate("MainWindow", "-1"))
self.label_2.setText(_translate("MainWindow", "稀释梯度为10倍，每孔上液量为"))
self.comboBox.setItemText(0, _translate("MainWindow", "100uL"))
self.comboBox.setItemText(1, _translate("MainWindow", "200uL"))
self.comboBox.setItemText(2, _translate("MainWindow", "1mL"))
self.label_4.setText(_translate("MainWindow", "最低稀释倍数的lg负对数为:"))
self.label_6.setText(_translate("MainWindow", "待检原液的TCID50的值为： 10"))
self.lineEdit_9.setText(_translate("MainWindow", "8"))
self.label_7.setText(_translate("MainWindow", "/mL"))
self.label_5.setText(_translate("MainWindow", "(如-2， -4)"))
self.lineEdit.setText(_translate("MainWindow", "8"))
self.lineEdit_2.setText(_translate("MainWindow", "8"))
self.lineEdit_3.setText(_translate("MainWindow", "7"))
self.lineEdit_4.setText(_translate("MainWindow", "3"))
self.lineEdit_5.setText(_translate("MainWindow", "1"))
self.lineEdit_6.setText(_translate("MainWindow", "0"))
self.lineEdit_7.setText(_translate("MainWindow", "0"))
self.lineEdit_8.setText(_translate("MainWindow", "0"))
self.label.setText(_translate("MainWindow", "请填写各稀释度的病变孔数（从低稀释倍数开始填）（自然数）"))
self.pushButton.setText(_translate("MainWindow", "计算 TCID50"))
self.pushButton_2.setText(_translate("MainWindow", "清除数据"))
self.label_3.setText(_translate("MainWindow", "每个稀释度重复数为:"))
self.groupBox_2.setTitle(_translate("MainWindow", "MOI 计算"))
self.lineEdit_12.setText(_translate("MainWindow", "-6"))
self.label_9.setText(_translate("MainWindow", "原液的TCID50的值为： 10"))
self.label_10.setText(_translate("MainWindow", "/mL"))
self.label_12.setText(_translate("MainWindow", "要计算MOI为"))
self.label_13.setText(_translate("MainWindow", "时，需要加入的病毒液的量为"))
self.lineEdit_13.setText(_translate("MainWindow", "1"))
self.label_14.setText(_translate("MainWindow", "细胞数量为："))
self.lineEdit_14.setText(_translate("MainWindow", "2"))
self.lineEdit_15.setText(_translate("MainWindow", "6"))
self.label_15.setText(_translate("MainWindow", "个"))
self.lineEdit_22.setText(_translate("MainWindow", "100"))
self.label_22.setText(_translate("MainWindow", "uL"))
self.label_23.setText(_translate("MainWindow", "X 10"))
self.pushButton_3.setText(_translate("MainWindow", "计算 MOI"))
self.label_24.setText(_translate("MainWindow", "此时，感染的细胞比例为："))
self.lineEdit_16.setText(_translate("MainWindow", "90"))
self.label_25.setText(_translate("MainWindow", "%"))
self.pushButton_4.setText(_translate("MainWindow", "清除"))
self.pushButton_6.setText(_translate("MainWindow", "填充"))
self.pushButton_5.setText(_translate("MainWindow", "退出"))
self.pushButton_7.setText(_translate("MainWindow", "帮助"))
self.pushButton_8.setText(_translate("MainWindow", "Copyright © 2020 SCAU"))
self.label_11.setText(_translate("MainWindow", "版本号：4.0.2"))
##与UI窗体类对应的业务逻辑类
import math
import sys
from PyQt5.QtWidgets import QApplication, QDialog, QMainWindow
#from PyQt5.QtGui import QPalette
from PyQt5.QtCore import Qt, pyqtSlot
import PyQt5.QtWidgets as qw
def jisuan(a, repeatNum):
# a = [] # 各CPE孔数
b = [] # 各非CPE孔数
a_acc = [] # 各CPE累积孔数
b_acc = [] # 各非CPE累积孔数
ab_acc = [] # 各孔累积和
a_ratio = [] # CPE累积孔数占比
# 将列表 字符串类型转换成整型
a = list(map(int, a))
# 显示输入的CPE孔数
print("\n CPE孔数为：\n ", a)
# 计算 个稀释度非CPE孔数
for i in range(0, 8, 1):
b.append(repeatNum - a[i])
print("\n 非CPE孔素为：\n ", b)
# 计算CPE累积孔数，从高稀释倍数到低稀释倍数
s = 0
for i in reversed(a):
s = s + i
a_acc.append(s)
a_acc.reverse()
print("\n CPE累积为：\n ", a_acc)
#计算非CPE累积孔数
s = 0
for i in b:
s = s + i
b_acc.append(s)
print("\n 非CPE累积为：\n ", b_acc)
# 计算总累积孔数
for m, n in zip(a_acc, b_acc):
ab_acc.append(m + n)
print("\n 孔累积和为：\n ", ab_acc)
# 计算各CPE累积孔数 占 总累积孔数（CPE累积和非CPE累积）的 比例
for m, n in zip(a_acc, ab_acc):
a_ratio.append(m / n)
for i, n in zip(range(8), a_ratio):
a_ratio[i] = '{0:.4f}'.format(a_ratio[i])
print("\n CPE累积占比为：\n ", a_ratio)
# 寻找大于等于最接近于50%的稀释度
# 寻找大于等于最接近于50%的稀释度 占率
gd50 = 0
for i, i2 in zip(reversed(a_ratio), range(8)):
if round(float(i)) >= 0.5:
gd50z = float(i)
gd50 = 8 - i2 - 1
break
# 寻找小于50%且最近于50%的稀释度 占率
for i in a_ratio:
if round(float(i)) < 0.5:
d50 = i
break
# 计算距离
s = (float(gd50z) - 0.5) / (float(gd50z) - float(d50))
print("\n 高于50%的稀释度为：{} 其比率为：{} ".format(gd50, gd50z))
# 计算lg(TCID50) =
tcid50 = -(gd50 + 1 + s)
print(tcid50)
# tcid50 = format(tcid50, '.4f')
return tcid50
def test_virus_volume(tcid50_value_init, volume_index):
if volume_index == 0:
tcid50 = tcid50_value_init - 1
elif volume_index == 1:
tcid50 = tcid50_value_init - math.log(5, 10)
else:
tcid50 = tcid50_value_init
return tcid50
def test_init_dilution_factor(tcid50_value, initFactor):
tcid50 = tcid50_value + 1 + initFactor
return tcid50
def virus_volume_need(tcid_fuzhi, cell_xi, cell_zhi, moi_value):
cell_count = cell_xi * (10 ** cell_zhi)
denominator = 0.7 * (10 ** (-tcid_fuzhi))
virus_volume = ((moi_value * cell_count) / denominator) * 1000 # 变微升
virus_volume = format(virus_volume, '.2f')
print(tcid_fuzhi)
print(cell_xi)
print(cell_zhi)
print(moi_value)
print(cell_count)
print(denominator)
print(virus_volume)
return str(virus_volume)
def infect_rate(moi_value):
p0 = math.e ** (-moi_value)
p1 = (1 - p0) * 100
p1 = format(p1, '.2f')
m = -math.log(p0, math.e)
print("p0", p0)
print("moi", m)
return str(p1)
class QmyDialog(QMainWindow):
def __init__(self, parent=None):
super().__init__(parent) # 调用父类构造函数，创建窗体
self.ui = Ui_MainWindow() # 创建UI对象
self.ui.setupUi(self) # 构造UI界面
self.setWindowTitle('TCID50计算器 4.0.1')
# self.setWindowIcon(QIcon("./favicon.icon"))
# self.setWindowIcon(QIcon(QPixmap(os.path.join('icons', "./favicon.icon"))))
# self.setFixedWidth(200)
# self.setFixedHeight(200)
#self.initUI()
# def initUI(self):
# # x轴位置，y轴位置,x轴长度，y轴长度
# #self.setGeometry(300, 300, 300, 300)
# self.setWindowTitle('TCID50计算器 4.0.1')
# self.setWindowIcon(QIcon("./favivon.icon"))
# self.show()
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_clicked(self):
a1 = a2 = a3 = a4 = a5 = a6 = a7 = a8 = 0
a = [a1, a2, a3, a4, a5, a6, a7, a8]
if self.ui.lineEdit.text() == "":
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'提示')
msgBox.setText(u"\n警告!\n请输入正确的数据\n请确认是否输入数据！")
# qw.QMessageBox.about(self, "警告", "请输入正确的数据！")
msgBox.exec_() # 模态对话框
else:
try:
a1 = float(self.ui.lineEdit.text())
a2 = float(self.ui.lineEdit_2.text())
a3 = float(self.ui.lineEdit_3.text())
a4 = float(self.ui.lineEdit_4.text())
a5 = float(self.ui.lineEdit_5.text())
a6 = float(self.ui.lineEdit_6.text())
a7 = float(self.ui.lineEdit_7.text())
a8 = float(self.ui.lineEdit_8.text())
except:
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'提示')
msgBox.setText(u"\n警告!\n请输入正确的数据\n请确认输入数据是否完整！")
msgBox.exec_() # 模态对话框
else:
# a_sum = a1 + a2 + a3 + a4 + a5 + a6 + a7 + a8
# 输入数据无异常，进行计算初试TCID50
a = [a1, a2, a3, a4, a5, a6, a7, a8]
repeatNum = int(self.ui.lineEdit_9.text())
print(repeatNum)
tcid50_value_init = float(jisuan(a, repeatNum))
# 考虑毒液测试体积，通常为100uL，index为0
volume_index = self.ui.comboBox.currentIndex()
tcid50 = test_virus_volume(tcid50_value_init, volume_index)
# 考虑起始稀释倍数,负对数通常为-1
initFactor = int(self.ui.lineEdit_10.text())
tcid50 = test_init_dilution_factor(tcid50, initFactor)
# 格式化输出
tcid50 = format(tcid50, '.4f')
self.ui.lineEdit_11.setText(str(tcid50))
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_3_clicked(self):
try:
tcid_fuzhi = float(self.ui.lineEdit_12.text()) # TCID50
cell_xi = float(self.ui.lineEdit_14.text()) # 细胞数量 系数
cell_zhi = float(self.ui.lineEdit_15.text()) # 细胞数量 指数
moi_value = float(self.ui.lineEdit_13.text()) # MOI值
except:
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'提示')
msgBox.setText(u"\n警告!\n请输入正确的数据\n请确认输入数据是否完整！\n")
# qw.QMessageBox.about(self, "警告", "请输入正确的数据！")
msgBox.exec_() # 模态对话框
else:
virus_volume_uL = virus_volume_need(tcid_fuzhi, cell_xi, cell_zhi, moi_value)
self.ui.lineEdit_22.setText(virus_volume_uL)
infect_rate100 = infect_rate(moi_value)
self.ui.lineEdit_16.setText(infect_rate100)
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_6_clicked(self):
tcid_last = self.ui.lineEdit_11.text()
self.ui.lineEdit_12.setText(tcid_last)
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_7_clicked(self):
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'帮助')
msgBox.setText(u"\n本款TCID50计算器采用Reed-Muench两氏法，可用于计算TCID50， EID50等.\
\nTCID50,median tissue culture infective dose,半数组织培养感染剂量,即指能在培养板孔或试管内引起半数细胞病变或死亡(cytopathic effect, CPE)所需的病毒量，用以表征病毒的滴度。\
\n\n注意：这里的病毒量不是具体的浓度，而是将原始样品稀释的倍数。\
比如，1 mL培养液，稀释1000倍后恰好导致50%的细胞感染，则TCID50为1000/mL。意思是每mL样品中含有的病毒导致50%细胞感染需要稀释的倍数。\
可根据测试病毒体积，起始稀释倍数等参数计算TCID50\
\n\n版本号：4.0.2\
\n更新日期：2020/11/26\
\n作者：艾强云\
\n联系方式(E-mail)：11542829382@qq.com"
)
msgBox.exec_() # 模态对话框
@pyqtSlot() # 确保弹窗只弹一次，否则会弹两次
def on_pushButton_8_clicked(self):
msgBox = qw.QMessageBox()
msgBox.setWindowTitle(u'版权信息')
msgBox.setText(u"\n华南农业大学兽医学院传染病教研室制品\n版权所有，侵权必究\n欢迎使用！\n \nCopyright © 2020 SCAU")
msgBox.exec_() # 模态对话框
if __name__ == "__main__": # 用于当前窗体测试
app = QApplication(sys.argv) # 创建GUI应用程序
# app_icon = QIcon("./favicon (1).ico")
# app.setWindowIcon(app_icon)
form = QmyDialog() # 创建窗体
form.show()
sys.exit(app.exec_())
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