SCPattern是一种基于经验贝叶斯方法来鉴定不同时间点的单细胞RNA-seq(scRNA-seq)数据表达模式,将其转化为表达模式(例如,Up-Up-Up-Up,Up-Up-Down-Down等。使用方法如下:
install.packages("devtools")
library(devtools)
install_github("lengning/SCPattern/package/SCPattern")
加载数据
data(SCPatternExData)
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模拟5个时间点的数据
CondVector <- rep(paste("t",1:5,sep=""),each=60)
Conditions <- factor(CondVector, levels=c("t1","t2","t3","t4","t5"))
对每个细胞进行标准化
Sizes <- MedianNorm(SCPatternExData,alternative = TRUE)
DataNorm <- GetNormalizedMat(SCPatternExData, Sizes)
计算基因的表达模式
res.multi <- SCPTest(SCPatternExData, CondVector, Sizes)
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