第三部分大意:
另一份由科学杂志发表的论文更直接地用到了RNA。在那篇论文中作者提到了由酶Cas13和另一种可以反向G-A突变效果的酶组成的碱基修正方法。虽然是针对RNA的碱基修正,但是效果上和DNA一样:通过将一种碱基换成另一种来改变蛋白质的组成和行为。
这些论文虽然各不相同,但都表明基因工程的工具正在迅速发展。尤基是Cas9的各种变体在不断用来测试CRISPR-Cas9的方法能否被改进。并用另一种叫作Cpf1的酶被尝试着用来代替Cas9和CRISPR一起实验。
这些发明碱基修正技术的研究人员甚至希望能够有机会研究出epigenomic修正技术。epigenomic 能够影响到一个细胞的类型。虽然目前看来还很遥远,但未来却拥有无限可能。
Another paper which was published in Science involves RNA more directly. The author describes a base editor made from Cas13 and anther enzyme which can reverse the effect of G to A mutation. Although it works on RNA, the effect is the same s DNA, substituing one base for another and thus changing the composition and activity of protein.
Though the papers are different, they demonstrate that the genetic engineering toolkit is developing rapidly. In particular, variants of Cas9 are used to test whether CRISPR-Cas9 approach can be improved. And another enzyme called Cpf1 are used to substitute Cas9 to test in conjunction with CRISPR.
These researchers of base editing even hope to reach epigenomic editing. epigenomic can influence the type of a cell. Although it seems a distant prospect, but the possibility is limitless.
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