荧光标记基团的激发和发射波长

荧光标记基团的激发和发射波长是广大科研工作者最关心的内容.
下面就我们大家常用的各种荧光基团数据参数提供给大家.

荧光染料 激发波长，nm 发射波长，nm
FITC 494 518
5-FAM 494 522
TAMRA 560 582
Rhodamine B 555 580
Cy3 550 570
Cy5 649 670

荧光分子 颜色 激发波长 发射波长
Alexa Fluor 350 Blue 346 nm 442 nm
Alexa Fluor 405 Blue 402 nm 421 nm
Pacific Blue Blue 410 nm 455 nm
DAPI Blue 340 nm 488 nm
AMCA Blue 350 nm 450 nm
Cascade Blue Blue 400 nm 420 nm
EviTag(TM) quantum dots - Lake Placid Blue (490 nm) Blue <400 nm 490 nm
Fluoroblue Blue 350 nm 450 nm
Image-Blue Blue 380 nm 465 nm
Marina Blue Blue 365 nm 460 nm
Pacific Blue Blue 410 nm 455 nm
Cy2 Green 491 nm 509 nm
Alexa Fluor 430 Green 434 nm 539 nm
Alexa Fluor 488 Green 495 nm 519 nm
Alexa Fluor 488 FluoroNanogold Green 495 nm 519 nm
Alexa Fluor 500 Green 503 nm 525 nm
Alexa Fluor 514 Green 518 nm 540 nm
FITC Green 490 nm 520 nm
Fluorescein Green 494 nm 518 nm
HNP Green 290 nm 509 nm
5-FAM Green 494 nm 522 nm
6-FAM Green 492 nm 517 nm
BODIPY FL Green 505 nm 513 nm
CMNB-caged Fluorescein Green 494 nm 518 nm
Cyanine 2 Green 492 nm 510 nm
DTAF Green 495 nm 520 nm
DTAF/FITC Green 495 nm 520 nm
dUTP-Biotin and Avidin FITC Green 495 nm 520 nm
dUTP-FITC Green 495 nm 520 nm
EviTag(TM) quantum dots - Adirondack Green (520 nm) Green <400 nm 520 nm
EviTag(TM) quantum dots - Catskill Green (540 nm) Green <400 nm 540 nm
FITC (Fluorescein) Green 495 nm 520 nm
Fluorescein-5-EX, succinimidyl ester (FSE) Green 495 nm 520 nm
Hi FITC Green 495 nm 520 nm
JOE Green 520 nm 548 nm
MFP488 Green 501 nm 523 nm
Oregon Green 488 Green 496 nm 524 nm
Oregon Green 514 Green 511 nm 530 nm
Qdot 525 Green 488 nm 515 nm
TET Green 521 nm 536 nm
CAL Fluor(R) Gold 540 Green 522 nm 544 nm
NL493 Green 493 nm 514 nm
GFP Green 395/475 nm 509 nm
Cy3 Yellow 548 nm 562 nm
Cy3B Yellow 558 nm 572 nm
Alexa Fluor 532 Yellow 531 nm 554 nm
Alexa Fluor 546 Yellow 556 nm 573 nm
Alexa Fluor 555 Yellow 555 nm 565 nm
R-Phycoerythrin Yellow 480, 546, 565 nm 578 nm
B-Phycoerythrin Yellow 546, 565 nm 575 nm
HNP/Fast Red TR Yellow 290 nm 562 nm
5-TAMRA Yellow 546 nm 574 nm
Atto 550 Yellow 554 nm 576 nm
CAL Fluor(R) Orange 560 Yellow 538 nm 559 nm
Cyanine 3 Yellow 550 nm 570 nm
DyLight 547 Yellow 547 nm 574 nm
EviTag(TM) quantum dots - Birch Yellow (580 nm) Yellow <400 nm 580 nm
EviTag(TM) quantum dots - Hops Yellow (560 nm) Yellow <400 nm 560 nm
HEX Yellow 535 nm 556 nm
NED Yellow 546 nm 575 nm
Qdot 565 Yellow <500 nm 565 nm
Quasar(R) 570 Yellow 548 nm 566 nm
R-Phycoerythrin (R-PE) Yellow 488 nm 575 nm
TRITC (Rhodamine) Yellow 550 nm 570 nm
VIC Yellow 538 nm 554 nm
NL557 Yellow 557 nm 574 nm
Phycoerythrin Yellow 480-566 nm 575 nm
Cy3.5 Orange 581 nm 596 nm
Alexa Fluor 568 Orange 578 nm 603 nm
ROX Orange 575 nm 602 nm
DyeMer 488/605 Orange 502 nm 609 nm
DyeMer 488/615 Orange 502 nm 615 nm
CAL Fluor(R) Red 590 Orange 569 nm 591 nm
CAL Fluor(R) Red 610 Orange 569 nm 610 nm
Cyanine 3.5 Orange 581 nm 596 nm
DyeMer 488/605 Orange 488 nm 605 nm
DyeMer 488/615 Orange 488 nm 615 nm
EviTag(TM) quantum dots - Fort Orange (600 nm) Orange <400 nm 600 nm
Lissamine Rhodamine Orange 570 nm 590 nm
MFP555 Orange 560 nm 585 nm
Qdot 585 Orange 532 nm 585 nm
Qdot 605 Orange <600 nm 605 nm
ROX Orange 575 nm 602 nm
R-Phycoerythrin-Texas Red (PE-Texas Red, ECD) Orange 480-565 nm 615 nm
Rhodamine Red-X Orange 570 nm 590 nm
TMR red Red 540 (520-560) nm 580 (570-620) nm
Cy5 Red 646 nm 664 nm
Cy5.5 Red 673 nm 692 nm
CypHer5E Red 644 nm 663 nm
Alexa Fluor 594 Red 590 nm 617 nm
Alexa Fluor 594 FluoroNanogold Red 590 nm 617 nm
Alexa Fluor 610 Red 612 nm 628 nm
Alexa Fluor 610–R-PE Red 496, 546, 565 nm 630 nm
Alexa Fluor 633 Red 632 nm 647 nm
Alexa Fluor 635 Red 633 nm 647 nm
Alexa Fluor 647 Red 650 nm 668 nm
Alexa Fluor 647–R-phycoerythrin Red 496, 546, 565 nm 668 nm
Alexa Fluor 660 Red 663 nm 690 nm
Texas Red Red 596 nm 620 nm
DyeMer 488/630 Red 502 nm 630 nm
Allophycocyanin Red 650 nm 660 nm
PI Red 535 nm 617 nm
Allophycocyanin (APC) Red 650 nm 660 nm
Allophycocyanin-Cyanine 5 Red 633 nm 670 nm
Allophycocyanin-Cyanine 5.5 Red 633 nm 690 nm
Atto 647 Red 645 nm 669 nm
CAL Fluor(R) Red 635 Red 618 nm 637 nm
Cyanine 5 Red 650 nm 670 nm
DyLight 647 Red 647 nm 673 nm
DyeMer 488/630 Red 488 nm 630 nm
Dyomics 647 Red 660 nm 673 nm
Lightcycler Red- 640 Red 625 nm 640 nm
PBXL-1 Red 545 nm 666 nm
PBXL-3 Red 614 nm 662 nm
PerCP (Peridinin Chlorophyll) Red 490 nm 675 nm
PerCP-Cy5.5 Red 488 nm 695 nm
Qdot 655 Red <620 nm 655 nm
Quantum Red Red 488 nm 660 nm
Quasar(R) 670 Red 647 nm 667 nm
R-Phycocyanin (R-PC) Red 614 nm 647 nm
R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red) Red 488 nm 660 nm
Sulforhodamine Red 585 nm 620 nm
Texas Red Red 596 nm 620 nm
Cyanine Q (Cyanine 5.18) Red 488 nm 670 nm
NL637 Red 637 nm 658 nm
Allophycocyanin Red 620-650 nm 660-670 nm
CytoCy5S Red 628 nm 638 nm
Alexa Fluor 680 Far-Red 679 nm 702 nm
Alexa Fluor 680–Allophycocyanin Far-Red 650 nm 702 nm
Alexa Fluor 680-R-phycoerythrin Far-Red 555 nm 700 nm
Alexa Fluor 700 Far-Red 702 nm 723 nm
Alexa Fluor 700–Allophycocyanin Far-Red 650 nm 723 nm
Alexa Fluor 750 Far-Red 749 nm 775 nm
Alexa Fluor 750–Allophycocyanin Far-Red 650 nm 775 nm
Allophycocyanin-Cyanine 7 Far-Red 633 nm 760 nm
Cyanine 5.5 Far-Red 678 nm 703 nm
Cyanine 7 Far-Red 710 nm 805 nm
IRDYE700DX Far-Red 689 nm 700 nm
IRDYE800 Far-Red 778 nm 795 nm
IRDYE800CW Far-Red 774 nm 800 nm
NIRD700 Far-Red 682 nm 701 nm
Qdot 705 Far-Red <700 nm 705 nm
Qdot 800 Far-Red <600 nm 800 nm
R-Phycoerythrin-Cyanine 5.5 Far-Red 488 nm 703 nm
R-Phycoerythrin-Cyanine 7 (PC7) Far-Red 488 nm 805 nm

荧光颜色    摩尔质量(g/mol) 吸收光谱(nm)    发射光谱(nm)    ε (M-1cm-1)

DyLight 350 紫色 874 353 432 15,000
DyLight 405 紫色 793 400 420 30,000
DyLight 488 绿色 1011 493 518 70,000
DyLight 550 黄色 982 562 576 150,000
DyLight 594 橙色 1078 593 618 80,000
DyLight 633 红色 1066 638 658 170,000
DyLight 650 红色 1008 654 673 250,000
DyLight 680 远红外 950 692 712 140,000
DyLight 755 近红外 1092 754 776 220,000
DyLight 800 近红外 1050 777 794 270,000

标记抗体的激发波长、发射波长
荧光素 荧光颜色 激发波长Ex（nm） 发射波长Em（nm）
Alexa Fluor 350 蓝色 346 422
FITC 绿色 495 519
Alexa Fluor 488 绿色 495 519
Cy3 橙色 514,552 570
Alexa Fluor 555 橙色 556 572
Rhodamine B 橙色 540 625
PE 橙色 496,564 578
PE-Cy3 橙色 488,532,561 578
APC 红色 650 660
Alexa Fluor 647 红色 647 666
PE-Cy5 红色 488,561,649 667
Cy5 红色 649 670
Cy7 红外 710,743 767
PE-Cy7 红外 488,561,743 785