高通量测序技术分类1

NGS sequencing method

NGS major types

Short-read sequencing

Short-read sequencing approaches fall under two broad categories: sequencing by ligation (SBL) and sequencing by synthesis (SBS).

There are several different **strategies used to generate clonal template **populations: bead-based, solid-state and DNA nanoball generation (FIG.1).

Template amplification strategies

Long-read sequencing

There are two main types of long-read tech�nologies: single-molecule real-time sequencing approaches and synthetic approaches that rely on existing short�-read technologies to construct long reads in silico.

Real-time and synthetic long-read sequencing approaches

NGS platforms

Summary of NGS platforms

参考资料：

1. Goodwin S , Mcpherson J D , Mccombie W R . Coming of age: ten years of next-generation sequencing technologies[J]. Nature Reviews Genetics, 2016, 17(6):333-351.