单变量

资料源自解螺旋
Long Non-Coding RNA (LncRNA) HOXA11-AS Promotes Breast Cancer Invasion and Metastasis by Regulating Epithelial-Mesenchymal Transition

• 疾 breast cancer
• 型 invasion and metastasis EMT
• 模 [method]
• 法 [method]
• 标 HOXA11

分子来源

Recent studies have revealed that HOXA11-AS could serve as an important biological marker and therapeutic target for other types of tumors [13–15]. However, there is no report about the role of lncRNA HOXA11-AS in breast cancer

In recent years, many studies have reported that lncRNA is involved in tumor invasion and metastasis by regulating EMT. Hao et al. [21] found that, as an important regulatory factor, lincRNA 01186 was involved in lung cancer cell invasion and metastasis by regulating EMT. Additionally, our results revealed interference in lncRNA HOXA11-AS could result in changes in the expressions of EMT molecular markers such as E-cadherin, N-cadherin, and Vimentin.

没做回复Rescue实验（没机制没法Rescue）

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实验方法

• Breast cancer tissue specimens
• Breast cancer cell strains
• Primer synthesis of siRNA and qRT-PCR interfering in
  HOXA11-AS
• Detection of HOXA11-AS expression via real-time
  fluorescence quantification PCR
• Cell culture and the transfection of human breast cancer
  cell strain
• Detection of breast cancer cell strain proliferation via MTT
  assay and clone formation assay
• Detection of cell cycle and apoptosis changes via flow
  cytometry
• Detection of cell migration and invasion capacity changes
  via Transwell experiment and wound healing assay
• Detection of EMT-related molecular marker expression
  changes via Western blotting and immunofluorescence
  experiment
• Research on the influence of HOXA11-AS on tumor cell
  metastasis capacity via experiment in vivo
• Immunohistochemistry

结果

• Up-regulated expression of HOXA11-AS in breast cancer
  tissue and cells
  表达差异
• Research on the biological function of HOXA11-AS in
  breast cancer cell via experiment in vitro
  体外实验
• Research on the influence of HOXA11-AS on breast cancer cell invasion and metastasis via in vivo experiment
  体内实验
• HOXA11-AS promotes breast cancer cell invasion and
  metastasis by regulating EMT

图片解析

A：癌症病人中表达
B：细胞株表达
C、D：转染沉默基因表达情况

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A、B：两个不同细胞株检测沉默基因后的生长曲线（MTT）
C：两个不同细胞株检测沉默基因后的克隆形成
D：对C图的统计

image.png
A：流式细胞检测沉默基因后凋亡情况
B：对A的统计
C、D：沉默基因后细胞周期变化
image.png

A/B/C/D：基因沉默后的侵袭、增殖能力变化

image.png
为什么转移后表达反而低了？？？图片中sh应该是si
image.png
N-cadherin或E-cadherin是EMT的biomarker
图片中sh应该是si
image.png image.png