刷了机，安装好conda环境后conda又疯了。
conda install -y samtools hisat2 bowtie2 bwa htseq bowtie
报错开始

Elapsed: 
An HTTP error occurred when trying to retrieve this URL.
HTTP errors are often intermittent, and a simple retry will get you on your way.
....
Solving environment: failed with initial frozen solve. Retrying with flexible solve.
PackagesNotFoundError: The following packages are not available from current channels:

先查询当前conda版本：conda -V
更新conda到最新版本：conda update -n base conda
再查一下conda版本：conda -V
然后更新一下所有：conda update --all

还是一样的报错

Collecting package metadata (current_repodata.json): done
# Solving environment: failed with initial frozen solve. Retrying with flexible solve.
# Solving environment: failed with repodata from current_repodata.json, will retry with next repodata source.
# Collecting package metadata (repodata.json): done
# Solving environment: failed with initial frozen solve. Retrying with flexible solve.
# Solving environment: -
# Found conflicts! Looking for incompatible packages. 

但 conda install -c conda-forge opencv=4.2.0可以被顺利执行并完成，

查询发现是清华镜像的问题（https://www.cnblogs.com/YlnChen/p/12710893.html），立马换中科大镜像， 没删除清华镜像，把中科大的放在最前面：

 - https://mirrors.ustc.edu.cn/anaconda/cloud/bioconda/linux-64
 - https://mirrors.ustc.edu.cn/anaconda/cloud/bioconda/

先少量安装尝试--最好一次放三个，一次性放太多，中间卡住就gg了
conda install -y samtools hisat2 bowtie2
成功！

如果上述不行，你可以参考上述链接尝试删除清华源，或修改频道 ：

conda config --add channels conda-forge
conda config --set channel_priority flexible

或者你可以用pip来安装 https://pypi.org/ ,如：

pip install samtools