一个整合的iso-seq和genome浏览器，可以把数据中和ref中相似的isoforms聚合到一起，方便查看是哪个对应哪个
https://www.biorxiv.org/content/biorxiv/early/2017/01/25/102905.full.pdf
Interactive analysis of Long-read RNA isoforms with Iso-Seq
Browser
Abstract
Background
Long-read RNA sequencing, such as Pacific Biosciences’ Iso-Seq method, enables
generation of sequencing reads that are 10 kilobases or even longer. These reads are
ideal for discovering splice junctions and resolving full-length gene transcripts without
time-consuming and error-prone techniques such as transcript assembly and junction
inference.
Results
Iso-Seq Browser is a Web-based visual analytics tool for long-read RNA sequencing
data produced by Pacific Biosciences’ isoform sequencing (Iso-Seq) techniques. Key
features of the Iso-Seq Browser are: 1) an exon-only web-based interface with zooming
and exon highlighting for exploring reference gene transcripts and novel gene isoforms,
- automated grouping of transcripts and isoforms by similarity, 3) many customization
features for data exploration and creating publication ready figures, and 4) exporting
selected isoforms into fasta files for further analysis. Iso-Seq Browser is written in
Python using several scientific libraries. The application and analyses described in this
paper are freely available to both academic and commercial users at https://github.com/
goeckslab/isoseq-browser
Conclusions
Iso-Seq Browser enables interactive genome-wide visual analysis of long RNA
sequence reads. Through visualization, highlighting, clustering, and filtering of gene
isoforms, ISB makes it simple to identify novel isoforms and novel isoform features such
as exons, introns and untranslated regions.
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