【文献速览】-Cell-by-Cell Deconstructi

Single-cell sequencing approaches offer exploration of tissue architecture at unprecedented resolution. These tools are especially powerful when deconvoluting highly specialized microenvironments, such as stem cell (SC) niches. Here, we review single-cell studies that map the cellular and transcriptional makeup of stem and progenitor niches and discuss how these high-resolution analyses fundamentally advance our understanding of how niche factors shape SC biology and activity. In-depth characterization of the blueprint of SC-niche crosstalk, as well as understanding how it becomes dysregulated, will undoubtedly inform the development of more efficient therapies for malignancies and other pathologies.

单细胞测序方法以前所未有的分辨率提供了组织结构的探索。这些工具在消除高度专门化的微环境(如干细胞(SC))时特别强大。在这里，我们回顾了绘制干细胞和祖细胞环境位的细胞和转录组成的单细胞研究，并讨论了这些高分辨率分析如何从根本上促进我们对环境位因素如何塑造SC生物学和活性的理解。深入描述SC-niche串话的蓝图，以及理解它如何变得失调，无疑将为开发更有效的治疗恶性肿瘤和其他病理学提供信息。