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进化树美化之Evolview--热图(2)

进化树美化之Evolview--热图(2)

作者: 周小钊 | 来源:发表于2020-09-08 21:14 被阅读0次

    上个教程中已经修改了进化树的分支颜色和标签颜色,接下来进行热图绘制

    数据准备

    我们可以从help界面查看我们需要输入的数据类型

    #heatmap
    !title  Example of heatmap  图例标题
    !showLegends    1           是否显示图例,1:显示,0:不显示
    !defaultStrokeColor gold
    !defaultStrokeWidth 2
    !colorgradient  blue, yellow,red  热图配色
    !colorgradientMarkLabel 0,5,10,15,20,25  颜色梯度值
    # -- heatmap column labels --
    !showHeatMapColumnLabel 1  热图表头显示
    !heatmapColumnLabels    e1,e2,e3,control  表头内容
    # -- heatmap --
    !heatmap    margin=2,colwidth=30,roundedcorner=2  热图内部信息
    # -- show data value
    !showdataValue  show=1,fontsize=12
    A   -2,3,5,0
    B   7,9,10,23
    C   13,15,19,8
    D   20,22,25,4
    

    根据示例数据将我们的数据修改好

    #heatmap
    !title  Expression of heatmap
    !showLegends    0
    #!defaultStrokeColor    gold
    #!defaultStrokeWidth    2
    !colorgradient  red,white,blue
    !showHeatMapColumnLabel 1
    # -- heatmap column labels --
    !showHeatMapColumnLabel 1
    !heatmapColumnLabels    HS1,HS2,HS3,HS4
    # -- heatmap --
    !heatmap    margin=1,colwidth=30,roundedcorner=5
    # -- show data value
    !showdataValue  show=0,fontsize=12,fontitalic=0,fontbold=1,textangle=-60,fontcolor=red
    AT1G03160.1 138.2662,119.4188,117.5653,131.9267
    AT4G17540.1 190.04398,130.73198,140.72378,150.41085
    Os06t0247800-01 22.3589,20.9118,19.7673,25.1028
    Os02t0738900-01 93.544,21.6591,18.8547,31.3205
    Os03t0732200-01 5.52722,4.83038,4.29929,3.81299
    Os10t0185200-00 83.1737,83.2791,71.5573,69.86103
    AT1G59610.1 40.8104,19.7877,21.998,34.7177
    AT1G10290.1 140.8104,119.7877,121.998,134.7177
    Os08t0425100-01 20.41115,39.6234,47.14298,63.02084
    Os12t0178700-01 48.51467,52.1542,52.7949,62.7378
    AT3G19720.1 124.4894,129.6265,127.352,132.6961
    Os01t0748000-00 103.341,352.318,236.861,293.542
    Os04t0129900-01 1.89585,8.67967,6.65843,2.84205
    Os03t0260000-01 131.2451,61.22141,49.86475,97.78848
    AT1G60500.1 29.84108,119.9479,125.1772,140.0944
    Os01t0920400-01 152.695,113.472,74.9321,61.9664
    AT2G14120.3 118.5393,229.62,230.3712,223.395
    AT4G33650.2 15.4361,255.7039,138.4035,133.0883
    Os04t0381000-01 1.81532,2.71541,4.09116,7.76133
    Os05t0556100-01 322.122,217.55541,18.8402,15.2791
    Os01t0681100-01 229.41612,249.59223,245.90516,125.5316
    AT3G61760.1 232.8752,251.1922,136.812,117.087
    AT5G42080.1 7.60426,3.105,8.66416,6.68762
    Os09t0572900-01 27.5529,94.32025,40.786299,40.476955
    AT1G14830.1 233.5316,235.4096,155.9334,164.4508
    Os03t0713100-01 5.59436,3.86999,5.44011,4.85916
    Os10t0567800-01 213.2103,218.9935,290.9288,225.216
    AT2G44590.3 29.6786,52.4297,272.53,278.289
    

    成品图:

    可以看到三个dataset已经在右边显示了,可以选择是否显示或者删除,也可以点笔的形状进行修改

    结果导出

    在上方有三个按钮,可以保存为png格式、pdf格式以及nwk格式

    左侧也有一个按钮,可以保存为多种格式

    将png格式保存到本地,可以看看效果

    fine!!!

    转载请注明:周小钊的博客>>>进化树美化之Evolview--热图(2)

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