文章目的：前列腺癌细胞可以释放细胞外囊泡到胞外环境中并且可以在尿液中被检测到，文章对9个前列腺癌病人与4个正常男性的尿液细胞外囊泡miRNA表达模式的差异进行了评估，提到了isomiRs这个事情。
文章信息
- 文献标题：Non‑invasive prostate cancer detection by measuring miRNA variants (isomiRs) in urine extracellular vesicles
- Doi：10.18632/oncotarget.8124
- 发表时间：March 16, 2016 Oncotarget
- 通讯作者：Irene Bijnsdorp 荷兰阿姆斯特丹V大学医学中心泌尿系
数据情况
A total of 28 million raw reads were obtained，Of these reads, about 50% could be mapped to the human genome with high confidence (Figure 1D) and with limited variation between the different samples. The reads were mapped to 32,000 individual loci of the human genome (hg19)
For all tested urine‑EV samples, in addition to miRNAs, which accounted for 44% of the aligned reads, we could classify all other groups of small RNAs. An average of 37% of the aligned reads mapped to the class of tRNAs, and 12% to rRNAs， Small RNA sequences that mapped to the other ncRNAs such as snRNAs, snoRNAs, piRNA and repeats were represented by less than 2% of the total mapped reads
The length distribution of the mapped miRNA sequences varied from 18 to 25 nt, of which most miRNAs were of 22 nt in length
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