GToTree做进化树

作者: 胡童远 | 来源:发表于2022-07-11 17:19 被阅读0次

    How to get phylogenetic tree from kraken or bracken output file?
    有人讨论如何用kraken的结果做进化树,
    尝试其中的一种方法,

    Mike Lee's tool GToTree
    It will take a list of NCBI accession IDs for each genome and create a phylogenetic tree out of them.

    Github: https://github.com/AstrobioMike/GToTree

    conda create -y -n gtotree -c conda-forge -c bioconda -c astrobiomike gtotree
    
    # /hwfssz5/ST_HEALTH/P18Z10200N0423/hutongyuan/analysis/fish/Abundance_kraken2/kraken_report
    # /hwfssz5/ST_HEALTH/P18Z10200N0423/hutongyuan/database/kraken2
    conda activate gtotree
    GToTree -a ncbi_accessions.txt -f fasta_files.txt -H Bacteria -D -j 4
    GToTree -h
    -----------------  EXAMPLE USAGE  -------------------
    GToTree -a ncbi_accessions.txt -f fasta_files.txt -H Bacteria -D -j 4
    

    [-a <file>] single-column file of NCBI assembly accessions
    [-g <file>] single-column file with the paths to each GenBank file
    [-f <file>] single-column file with the paths to each fasta file
    [-A <file>] single-column file with the paths to each amino acid file

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