03.查看不同粒度下的分群走向

在对粒度进行选择或注释存在疑问时，往往需要对粒度进行调整，为方便的查看不同粒度下的细胞走向，使用以下代码进行可视化。

rm(list = ls())
library(Seurat)
# devtools::install_github('satijalab/seurat-data')
library(SeuratData)
library(ggplot2)
library(patchwork)
library(dplyr)
load(file = 'basic.sce.pbmc.Rdata')
DimPlot(pbmc, reduction = 'umap', 
        label = TRUE, pt.size = 0.5) + NoLegend()
sce=pbmc
#先执行不同resolution 下的分群
library(Seurat)
library(clustree)
sce <- FindClusters(
  object = sce,
  resolution = c(seq(.1,1.6,.2)) #起始粒度，结束粒度，间隔
)
clustree(sce@meta.data, prefix = "RNA_snn_res.")

参考来源：
https://mp.weixin.qq.com/s/WRhMC3Ojy1GWYfLS_4vSeA

鸣谢：
I thank Dr.Jianming Zeng(University of Macau), and all the members of his bioinformatics team, biotrainee, for generously sharing their experience and codes.

问题交流：
Email: xuran@hrbmu.edu.cn