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10X scrna-seq||Loupe 软件中heatmap几

10X scrna-seq||Loupe 软件中heatmap几

作者: 周运来就是我 | 来源:发表于2019-07-31 07:06 被阅读25次

我们发现在loupe种一个细胞群的红色基因(log2 fold-change)基本在一起,那么它是如何做对的呢?

Question 1 : Can I customize the colors in the gene expression heatmap shown in Loupe Cell Browser?

Answer: At this time there is no way to customize the colors shown in the gene expression heatmap in Loupe Cell Browser (version 2.0.0 or earlier).

Another option is to create custom heatmaps in Python or R using the Cell Ranger pipeline output. The cellrangerRkit tutorial shows an example of how to create a similar heatmap in R (see Section 4.2, Figure 5).

Question 2: Which genes are displayed in the Loupe Cell Browser heatmap? How can I export the list of genes displayed?

Answer: In Loupe Cell Browser (version 2.0.0), the heatmap is a compact display of a subset of differentially expressed genes per cluster. Specifically, the gene list is the union of the top 120/N upregulated genes for each cluster ranked by log2 fold-change (N=total number of clusters).

The gene names are on the plot when you export the heatmap. However, as of version 2.0.0, there is no 1-click function to export the associated information for the subset of heatmap genes.

10X||heatmap

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