SSR 中性检测 PopGene

作者: 董八七 | 来源:发表于2019-08-02 16:53 被阅读16次

    Xiao Y, Cai D, Yang W, et al (2012) Genetic structure and linkage disequilibrium pattern of a rapeseed (Brassica napus L.) association mapping panel revealed by microsatellites. Theor Appl Genet 125:437–447. doi: 10.1007/s00122-012-1843-5

    中性检测

    使用PopGene 1.31版软件(Yeh等人1999)中实施的Manly算法(1985年)对各组和亚组进行了Ewens-Watterson的中立性测试(Ewens 1972;Watterson 1978),以调查整个基因座的选择性中立性。这是为了测试观察到的纯合子(计算为等位基因频率的平方和)是否明显高于或低于中性预期下的模拟预期纯合子,并可以提示选择是否在人群中的特定基因座上进行(Ewens 1972;Watterson 1978)。

    数据

    /* Diploid alphabetic data of 1 populations each with varying records (genotypes) & 21 loci */
    Number of populations = 1
    Number of loci = 21
    Locus name: AAT-1 AAT-2 AAT-3 ACO ADH DIA-1 DIA-3 EST-2 GDH G6P HA IDH MDH-1 MDH-2 MDH-3 MDH-4 PEP-1 PEP-2 PGI-2 PGM SPG-2
    
    AA AA AA AA AA AA AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AB BB A3 AA AB BB AA AA AA AA AA AA AA AA AA AB AA AA
    AA AA AA AA BC AC AA AB AB AB AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA BB CC AA BB AB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA AB AC AA BB AA AA AA AA AA AA AA AC AA AA AA AB AA
    AA AA AA AB AB AC AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
    AB AA AA AA BC AC AA AB AB AA AA AA AA AB AA AB AA AA AA AA AA
    AA AA AA AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA AC AA AA
    AA AA AA AA AA BC AA AB AA AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AB BC BC AA BB AB AA AA AA AA AB AA AA AA AA AA AC AA
    AA AA AA AB AC AB AA BB BB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA AB AC AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA AA BC AB AB AA AA AA AA AA AA AA AA AA AA AC AA AA
    AA AA AA AB AA AC AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AA BB BB AA AA AA AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AA AC AC AA BB AB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AB BB BC AA BB AA AC AA AA AA AA AA AA AA AA AC AD AA
    AA AA AA AA AB BC AA AB AB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA BB BC AA BB AA AA AA AA AA AA AA AB AA AA AA AC AA
    AA AA AA AA AA BC AA AB AB AA AA AA AA AA AA BC AA AA AA AA AA
    AA AA AA AA AB BC AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AA BD BC AA AB AB AA AA AA AA AA AA AA AA AA AC AE AA
    AA AA AA AB CC BB AA BB AA AA AA AA AA AA AA AA AA AA AA AB AA
    AA AA AA AA BC BB AA AB AB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AB CC BB AA AA AB AA AA AA AA AA AA AA AA AA AA AB AA
    AA AA AA AA BB BC AA AB AB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA AB BC AA AA BB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AB AC AB AA BB AA AA AA AA AA AA AA AA AA AA AA AB AA
    AA AA AA AB BC BB AA BB BB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AB AB AB AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA BB AB AA AA AA AA AA AA AA AA AA BB AA AA AA AA AA
    AA AA AA AA AC BC AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AA CC BC AA AB AB AA AA AA AA AA AA AB AA AA AA AC AA
    AA AA AA AA BB AC AA BB AA AA AA AA AA AA AA AA AA AA CC AA AA
    AA AA AB AA BE BB AA BB AB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AA AB BC AA AB AA AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA BB AA CC AA AB AB AA AA AA AA AA AA BC AA AA AA AA AA
    AA AA AA AB AC BC AA BB BB AA AA AA AA AA AA AA AA AA AA AA AA
    AA AA AA AB AA BB AA AC AB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AB BB AC AA BB AA AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AA AC AC AA AA AB AA AA AA AB AA AA AB AA AA AA AA AA
    AA AA AA AA AA AB AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
    AA AA AA AA BC BC AA BB AB AA AA AA AA AA AA AB AA AA AA AC AA
    

    popgene

    加载数据

    file→load data→co-dominant marker datayou→选择确定。

    分析

    co-dominant→diploid data:

    勾选
    注意这里一定要选分层结构(hirarchical structure),然后勾选neutrality test。然后一路确定。

    结果

    结果

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