File->open,或者快捷键ctrl+o,打开所要编辑的图片
Image->Type->8-bit,灰度变换
Edit->Invert
Image->Adjust->Threshold,灰度阈值设定,拖动完成后点击Apply
Process->Binary-Fill Holes填补细胞核的空隙
Process->Binary-Watershed打断细胞核的重叠部分
Process->Noise->Despekle,去除离群点
Process->Smooth,图像平滑
Analyze->Analyze Particles,计数
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