Single cell RNA-seq

作者: zhoujj2013 | 来源:发表于2018-02-24 22:01 被阅读90次

    10X platform
    https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest

    https://scanpy.readthedocs.io/en/latest/examples.html

    http://cf.10xgenomics.com/supp/cell-exp/megacell_tutorial-1.0.1.html

    http://cf.10xgenomics.com/supp/cell-exp/cellrangerrkit-PBMC-vignette-knitr-2.0.0.pdf

    http://satijalab.org/seurat/

    https://portals.broadinstitute.org/single_cell

    Can not get long noncoding transcripts

    https://academic.oup.com/bfg/advance-article/doi/10.1093/bfgp/elx035/4604806

    know the single cell sequencing processes

    https://community.10xgenomics.com/t5/10x-Blog/Single-Cell-RNA-Seq-An-Introductory-Overview-and-Tools-for/ba-p/547

    resources

    https://hemberg-lab.github.io/scRNA.seq.datasets/mouse/tissues/
    https://hemberg-lab.github.io/scRNA.seq.course/introduction-to-single-cell-rna-seq.html#challenges
    https://github.com/seandavi/awesome-single-cell
    https://github.com/diazlab/SCell
    https://www.10xgenomics.com/resources/publications/?solution=single-cell&research-area=cancer-research
    http://science.sciencemag.org/content/early/2016/07/27/science.aad7038.full

    standard analysis

    https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest
    http://go.10xgenomics.com/software-demos/single-cell-gene-expression

    analysis method

    https://www.nature.com/articles/s41467-017-02554-5
    https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1305-0
    https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0492-3
    http://ilab.hawaii.edu:8116/?state_id=f88ab815a1831b34&tab=info
    https://www.nature.com/articles/nmeth.4463
    https://www.nature.com/articles/s41593-017-0056-2
    http://www.pnas.org/content/114/37/E7786

    visualization

    https://shiny.rstudio.com/gallery/genome-browser.html

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