文献信息:
标题:Metabolic cross-feeding in imbalanced diets allows gut microbes to improve reproduction and alter host behaviour
中文:在不平衡的饮食中进行代谢交叉喂养可以使肠道微生物改善繁殖和改变宿主行为
杂志:NC
时间:2020.8.25
摘要:
共生细菌对宿主的影响来自于复杂的微生物-饮食-宿主的相互作用。因此,绘制肠道微生物群落的代谢相互作用是理解微生物群落如何影响宿主的关键。在这里,我们使用了跨学科的方法,包括同位素分解代谢组学,表明在黑腹果蝇,醋酸杆菌(Ap)和植物乳杆菌(Lp)中建立了一种共营养关系,以克服有害的宿主饮食,并确定Ap是改变宿主觅食决定的细菌。具体来说,我们发现Ap利用Lp产生的乳酸来提供Lp所必需的氨基酸,使其能够在不平衡的饮食中生长。乳酸对于Ap改变苍蝇对蛋白质的胃口也是必要和充分的。我们的数据显示,肠道细菌群落利用新陈代谢的相互作用来适应有害的宿主饮食。这些相互作用也确保了微生物组代谢产物的持续流动,以改变繁殖和宿主的行为。
Ap/Lp biassociation reduces yeast appetite and promotes bacterial growth independently of dietary Ile content.
In vitro growth measurements show that Lp can overcome its auxotrophy for Ile when co-cultured with Ap.
Ap synthesises and secretes isoleucine in the presence of Lp.
Lactate production by Lp is necessary and sufficient to reduce yeast appetite in the presence of Ap.
Ap synthesises Ile from lactate through an aspartate-derived biosynthetic pathway.
Bacterial amino acid supply is unlikely to explain the effect of gut bacteria on behaviour.
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