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2020-12-08 IQtree学习笔记

2020-12-08 IQtree学习笔记

作者: 云若蓝 | 来源:发表于2020-12-08 21:12 被阅读0次

    IQtree笔记

    根据windows软件phylosuite跑的IQtree代码,修改后在服务器中跑
    phylosuite原代码

    Command: D:\software\PhyloSuite_v1.2.2_Win64_with_plugins\PhyloSuite_v1.2.2_Win\PhyloSuite\plugins\iqtree-1.6.8-Windows\bin\iqtree.exe -s D:/software/PhyloSuite_v1.2.2_Win64_with_plugins/PhyloSuite_v1.2.2_Win/PhyloSuite/myWorkPlace/GenBank_File/files\IQtree_results\accD_mafft\accD_mafft.fas -st CODON11 -m TEST -alrt 1000 -bb 5000 -bcor 0.90 -wbt -nt AUTO
    

    修改后代码

    Command: bin\iqtree.exe -s accD_mafft.fas -st CODON11 -m MFP -alrt 1000 -bb 5000 -bcor 0.90 -T AUTO -wbt
    
    代码说明

    -m MFP(版本1.5.4之后,且为默认参数)和 -m TESTNEW(版本1.5.3之前)是不同版本的同义代码

    20201218日更新代码:IQtree批量建树

    /root/anaconda3/bin/iqtree -s /home/xmz/data/IQtree/单个基因分别建树 -st CODON11 -m MFP -alrt 1000 -bb 5000 -bcor 0.90 -T AUTO -wbt
    
    图片.png

    -alrt SH-aLRT检验,检验树的拓扑结构可信度,作者推荐大数据运算时使用。

    The branches of the resulting .treefile will be assigned with both SH-aLRT and
    UFBoot support values, which are readable by any tree viewer program like FigTree,
    Dendroscope or ETE. You can also look at the textual tree fgure in .iqtree fle:
    TIP: Starting with version 1.5.4, -m MFP is the default behavior. Thus, this run is equivalent to iqtree -s example.phy.


    图片.png

    此外,还可以合并树


    图片.png 图片.png 图片.png

    目前我还未知该合并方式和ASTRAL有何差别。
    -nt AUTO在最新版本里改为-T AUTO

    IQtree运行结果相关

    图片.png 图片.png

    来源

    https://www.jianshu.com/p/df234ca0de71

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