制作BW文件的时候需要去除位于黑名单区域的信号

我看到一个biostars论坛提问很有趣

The code I used;

$ computeMatrix scale-regions -S Sample1.bw Sample2.bw Sample3.bw -R genes.bed -a 5000 -b 5000 -o Samples.matrix --regionBodyLength 15000 --skipZeros
$ plotProfile -m Samples.matrix -out Samples.profile.png --perGroup

结果出图很诡异：

enter image description here

How can I remove them?

第一个回答我觉得就是对的：

I expect you have some outlier bin in one gene (just remove that gene, though in the future you'll want to blacklist that region when making bigWig files) that will be apparent in the heatmap.

如果不想在bw文件生成环节修改，也可以

If you want to actually edit the heatmap then don't bother with --outFileNameMatrix, just gunzip the output from computeMatrix, remove the problematic line, change the group_boundaries so they limits are correct, and then gzip the results back up.

In general, it's probably simpler to just figure out where the problem region is and remake the bigWig files with that region blacklisted.