文献阅读-LncRNA MALAT-1在很多癌症中均为Bioma

作者: PriscillaBai | 来源:发表于2019-05-19 12:34 被阅读44次

原文：The Long Noncoding RNA MALAT-1 is A Novel Biomarker in Various Cancers: A Meta-analysis Based on the GEO Database and Literature
2016年发表在journal of cancer, IF= 3.2

OS：over survival
RFS: recurrence-free survival

背景介绍

MALAT-1在很多癌症中均过表达，表明它可能作为biomarker。因此，作者收集了GEO数据库中的多组数据，用pooled odds ratio (OR) 和Hazard ratio (HR)表明biomarker的可信度。

数据分析流程

1）用单因素cox分析计算HR值和它们对应的95%置信区间，画生存分析曲线
2）计算得到数据的OS，DFS，RFS，临床诊断标准和其它因素。对于OS，RFS和DFS，进行异质性检测，加入两个数据之间存在很大的异质性，用随机影响模型和亚组分析
3）对于已有OS和DFS的数据，计算funnel plot
4）对于有临床病理特征的数据，将OR值和异质性检测MALAT-1表达和其它临床信息的关系

结果

作者对每个数据集进行了质量评估，根据Steels的方法。质量越高表明方法更靠谱。

实验流程

作者分析了MALAT-1和survival（OS/RFS）之间的关系，绘制了生存分析曲线，得到了HR，95%CI（表1）。计算了MALAT-1和临床信息之间的OR值。

根据来源地区（亚洲，欧洲）和来源（published articles, GEO datasets）进行分析
MALAT-1 was found to be significantly associated with the OS in cancer patients based on data from published articles (pooled HR: 3.97, 95%CI: 1.68-9.42), but not based on data from the GEO database；Subgroups were analyzed based on the data resource and by considering the presence of heterogeneity. Founding association was significant between MALAT-1 and the OS of cancer patients in Asian (pooled HR: 2.06, 95%CI: 1.62-2.61) but not western (pooled HR: 1.02, 95%CI: 0.86-1.21) populations.