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相似性热图复现

相似性热图复现

作者: monkey_study | 来源:发表于2022-12-01 16:19 被阅读0次

    原图

    image.png

    复现结果

    image.png

    复现代码

    1. 载入R包
    library(ComplexHeatmap)
    library(ggplot2)
    library(dplyr)
    library(circlize)
    
    1. 构造数据
    data=data.frame(cell_type=c('cDC3_LAMP3','cDC2_CD1C','cDC1_CLEC9A'),
                    mregDC=c(0.95,0.05,0.10))
    mat=data%>%tibble::column_to_rownames('cell_type')
    col_fun=circlize::colorRamp2(c(0,1),c("#F5E2CE","#EE2928"),space = 'RGB')
    mat=as.matrix(mat)
    

    3.画图

    ## 文本绘制
    layer_fun <- function(j, i, x, y, w, h, f) {
      grid.text(round(pindex(mat, i, j), 2),
                x, y, gp = gpar(fontsize = 10))
    }
    # 可视化
    Heatmap(mat
            ,col = col_fun
            ,layer_fun = layer_fun
            ,rect_gp = gpar(col = "white", lwd = 2) #方格的分割
            ,cluster_rows = F
            ,column_names_side = 'bottom'
            ,column_names_rot = 0
            ,column_names_gp = gpar(
              col='black'
              ,fontsize=15
              
            )
            ,row_names_side = 'left' #行名在左
            ,row_names_centered = T  #行名居中
            ,column_names_centered = T #列名居中
            ,row_names_gp = gpar(
              col='black'
              ,fontsize=15
              
            )
            ,border = T
            ,border_gp = gpar(
              col='white'
              ,alpha=0.5
              
            )
            , width = unit(2, "cm") #热图主体
            ,height = unit(8, "cm")
    #图例
            ,heatmap_legend_param = list(  
              at=round(seq(0,1,0.25),3)
              ,labels=c('0.00','0.25','0.50','0.75','1.00')
              ,title='Predicted\nSimilarity'
              ,title_gp=gpar(fontface=1,fontsize=12)
              ,legend_height=unit(6,'cm')
              ,gird_width=unit(2/3,'cm')
              
            )
            )
    

    参考:
    R 数据可视化 —— 聚类热图 ComplexHeatmap(四)图例 - 简书 (jianshu.com)
    R 数据可视化 —— 聚类热图 ComplexHeatmap(一) - 简书 (jianshu.com)

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