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史上最全workshop-关于NGS数据处理的-所有ppt可以下

史上最全workshop-关于NGS数据处理的-所有ppt可以下

作者: 热衷组培的二货潜 | 来源:发表于2018-09-05 15:40 被阅读13次
    image.png

    链接:https://qcb.ucla.edu/collaboratory/workshops/bsseq/#toggle-id-3

    发现来源于生信技能树:
    http://www.biotrainee.com/thread-1511-1-1.html

    2018-9-8
    https://github.com/Zhang-lab

    image.png

    下面这个已经有预印版本的paper了
    paper链接
    https://www.biorxiv.org/content/early/2018/09/04/407312.full.pdf+html

    image.png

    github 链接
    https://github.com/maxplanck-ie/snakepipes

    image.png

    已发表的文献的所有分析以及可视化脚本(主要学习可视化方面,比较是发表过了的theme)
    https://github.com/sung/CSMD1.paper

    image.png

    主要是参考下这两个脚本

    image.png

    主题代码

    # code from https://rpubs.com/Koundy/71792
    
    #theme_Publication <- function(base_size=14, base_family="helvetica") {
    theme_Publication <- function(base_size=14, base_family="") {
          library(grid)
          library(ggthemes)
          (theme_foundation(base_size=base_size, base_family=base_family)
           + theme(plot.title = element_text(face = "bold",size = rel(1.3), hjust = 0.5),
                text = element_text(),
                panel.background = element_rect(colour = NA),
                plot.background = element_rect(colour = NA),
                #panel.border = element_rect(colour = NA),
                axis.title = element_text(face = "bold",size = rel(1.2)),
                axis.title.y = element_text(angle=90,vjust =2),
                axis.title.x = element_text(vjust = -0.2),
                axis.text = element_text(size=rel(1.1)), 
                axis.line = element_line(colour="black"),
                axis.ticks = element_line(),
                panel.grid.major = element_line(colour="#f0f0f0"),
                panel.grid.minor = element_blank(),
                legend.key = element_rect(colour = NA),
                #legend.background = element_rect(colour = 'black', fill='grey',linetype='dashed'),
                legend.position = "right",
                #legend.direction = "horizontal",
                legend.key.size= unit(0.7, "cm"),
                legend.margin = unit(0.1, "mm"),
                legend.title = element_text(face="bold.italic",size=rel(1)),
                legend.text = element_text(size = rel(0.9),family = "sans"),
                plot.margin=unit(c(10,5,5,5),"mm"),
                strip.background=element_rect(colour="#f0f0f0",fill="#f0f0f0"),
                strip.text = element_text(face="bold",size=rel(1.1))
              ))
          
    }
    
    scale_fill_Publication <- function(...){
          library(scales)
          discrete_scale("fill","Publication",manual_pal(values = c("#386cb0","#fdb462","#7fc97f","#ef3b2c","#662506","#a6cee3","#fb9a99","#984ea3","#ffff33")), ...)
    
    }
    
    scale_colour_Publication <- function(...){
          library(scales)
          discrete_scale("colour","Publication",manual_pal(values = c("#386cb0","#fdb462","#7fc97f","#ef3b2c","#662506","#a6cee3","#fb9a99","#984ea3","#ffff33")), ...)
    
    }
    
    
    # code from https://gist.github.com/adamwespiser/3077780
    theme_publish <- function(base_size = 12) {
      structure(list(
        axis.line =         element_blank(),
        axis.text.x =       theme_text(size = base_size * 0.8 , lineheight = 0.9, vjust = 1),
        axis.text.y =       theme_text(size = base_size * 0.8, lineheight = 0.9, hjust = 1),
        axis.ticks =        theme_segment(colour = "black", size = 0.2),
        axis.title.x =      theme_text(size = base_size, vjust = 1),
        axis.title.y =      theme_text(size = base_size, angle = 90, vjust = 0.5),
        axis.ticks.length = unit(0.3, "lines"),
        axis.ticks.margin = unit(0.5, "lines"),
        
        legend.background = theme_rect(colour=NA), 
        legend.key =        theme_rect(colour = "grey80"),
        legend.key.size =   unit(1.2, "lines"),
        legend.text =       theme_text(size = base_size * 0.8,family = "sans"),
        legend.title =      theme_text(size = base_size * 0.8, face = "bold", hjust = 0),
        legend.position =   "right",
        
        panel.background =  theme_rect(fill = "white", colour = NA), 
        panel.border =      theme_rect(fill = NA, colour="grey50"), 
        panel.grid.major =  element_blank(),
        panel.grid.minor =  element_blank(),
        panel.margin =      unit(0.25, "lines"),
        
        strip.background =  theme_rect(fill = "grey80", colour = "grey50"), 
        strip.text.x =      theme_text(size = base_size * 0.8),
        strip.text.y =      theme_text(size = base_size * 0.8, angle = -90),
        
        plot.background =   theme_rect(colour = NA),
        plot.title =        theme_text(size = base_size * 1.2),
        plot.margin =       unit(c(1, 1, 0.5, 0.5), "lines")
      ), class = "options")
    }
    

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