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ggpubr进行“paper”组图合并,也许比PS,AI更简单

ggpubr进行“paper”组图合并,也许比PS,AI更简单

作者: 生信补给站 | 来源:发表于2019-08-08 11:03 被阅读14次

    多个图形进行组图展示,可以既展示一个“事情”的多个角度,也可以进行异同的比较,同时也是发表论文所必须的。

    可以利用PS或者AI进行处理,但是图形的大小,位置,布局,字体等的调整也不是一个小工程。本文利用R包 - ggpubr 函数从0开始介绍组图的合并方式,也许...... 比AI或者PS更简单易学呢。

    基础函数进行组图合并可参考[R |绘图边距及布局

    一 载入数据,R包

    加载函数包及数据集

    #install.packages("ggpubr")
    library(ggpubr)
    # ToothGrowth数据集
    data("ToothGrowth")
    head(ToothGrowth)
       len supp dose
    1  4.2   VC  0.5
    2 11.5   VC  0.5
    3  7.3   VC  0.5
    4  5.8   VC  0.5
    5  6.4   VC  0.5
    6 10.0   VC  0.5
    

    # mtcars 数据集

    data("mtcars")
    mtcars$name <- rownames(mtcars)
    mtcars$cyl <- as.factor(mtcars$cyl)
    head(mtcars[, c("name", "wt", "mpg", "cyl")])
                                   name    wt  mpg cyl
    Mazda RX4                 Mazda RX4 2.620 21.0   6
    Mazda RX4 Wag         Mazda RX4 Wag 2.875 21.0   6
    Datsun 710               Datsun 710 2.320 22.8   4
    Hornet 4 Drive       Hornet 4 Drive 3.215 21.4   6
    Hornet Sportabout Hornet Sportabout 3.440 18.7   8
    Valiant                     Valiant 3.460 18.1   6
    
    

    创建用于图形组合的图:
    #箱线图

    Box_plot <- ggboxplot(ToothGrowth, x = "dose", y = "len",color = "dose", palette = "jco")
    
    img

    #点图

    Dot_plot <- ggdotplot(ToothGrowth, x = "dose", y = "len",                 color = "dose", palette = "jco", binwidth = 1)
    

    [图片上传失败...(image-c31ccb-1565233425806)]

    #有序条形图

    Bar_plot <- ggbarplot(mtcars, x = "name", y = "mpg",          
    fill = "cyl",               # change fill color by cyl         
    color = "white",            # Set bar border colors to whit          
    palette = "jco",            # jco journal color palett. see ?ggpar  
    sort.val = "asc",           # Sort the value in ascending order      
    sort.by.groups = TRUE,      # Sort inside each group         
    x.text.angle = 90           # Rotate vertically x axis texts        
    ) + font("x.text", size = 8)
    
    img

    # 散点图

    Scatter_plots <- ggscatter(mtcars, x = "wt", y = "mpg",                
    add = "reg.line",               # Add regression line                
    conf.int = TRUE,                # Add confidence interval               
    color = "cyl", palette = "jco", # Color by groups "cyl"                
    shape = "cyl"                   # Change point shape by groups "cyl"     
    ) +  stat_cor(aes(color = cyl), label.x = 3)       # Add correlation coefficien
    
    img

    二 图形组合

    使用ggpubr包的函数ggarrange()中在一页上进行组合展示

    1)ToothGrowth数据集的箱线图,点图 组合展示

    ggarrange(Box_plot, Dot_plot,labels = c("A", "B"),ncol = 2, nrow = 1)
    
    img

    #图的边缘放置共同的唯一图例:common.legend = TRUE参数

    ggarrange(bxp, dp, labels = c("A", "B"),         common.legend = TRUE, legend = "bottom")
    
    img

    2)mtcars 数据集的条形图,散点图组合展示

    figure <- ggarrange(Scatter_plots, Bar_plot + font("x.text", size = 10),ncol = 1, nrow = 2)
    

    添加图形的注释信息(标题,副标题,坐标轴,字体,颜色等)

    annotate_figure(figure,
                    top = text_grob("Visualizing mpg", color = "red", face = "bold", size = 14),
                    bottom = text_grob("Data source:  mtcars data set", color = "blue",
                                       hjust = 1, x = 1, face = "italic", size = 10),
                    left = text_grob("Figure arranged using ggpubr", color = "green", rot = 90),
                    right = "Here )!",
                    fig.lab = "Figure 1", fig.lab.face = "bold"
                    )
    
    img

    3)ggarrange()函数更改绘图的列/行跨度

    散点图在第一行跨两列,箱形图和点图并于第二行

    ggarrange(Scatter_plots,                # First row with scatter plot
             ggarrange(Box_plot, Dot_plot, ncol = 2, labels = c("B", "C")), # Second row with box and dot plots
             nrow = 2,
             labels = "A"                     # Labels of the scatter plot
             )
    
    img

    4)利用NULL构建空白图

    示例:绘制具有边际密度图的散点图

    绘制主要散点图

    Scatter_plots <- ggscatter(iris, x = "Sepal.Length", y = "Sepal.Width",
                   color = "Species", palette = "jco",
                   size = 3, alpha = 0.6)+ border()
    

    上侧,右侧添加密度图

    xplot <- ggdensity(iris, "Sepal.Length", fill = "Species",
                      palette = "jco")
    yplot <- ggdensity(iris, "Sepal.Width", fill = "Species",
                      palette = "jco")+ rotate()
    # 设置主题
    yplot <- yplot + clean_theme()
    xplot <- xplot + clean_theme()
    # 通过width和height参数调整图的大小
    # 利用NULL设置空白图
    ggarrange(xplot, NULL, Scatter_plots, yplot,
             ncol = 2, nrow = 2,  align = "hv",
             widths = c(2, 1), heights = c(1, 2),
             common.legend = TRUE)
    
    img

    5)添加统计图表及文本信息

    绘制变量“Sepal.Length” 的密度图以及描述性统计(mean,sd,...)的汇总表。

    Sepal.Length密度图

    density.p <- ggdensity(iris, x = "Sepal.Length",
                          fill = "Species", palette = "jco")
    
    #Sepal.Length描述性统计
    stable <- desc_statby(iris, measure.var = "Sepal.Length",
                         grps = "Species")
    stable <- stable[, c("Species", "length", "mean", "sd")]
    
    #设置table的主题
    stable.p <- ggtexttable(stable, rows = NULL,
                           theme = ttheme("mOrange"))
    
    #  text 信息
    text <- paste("iris data set gives the measurements in cm",
                 "of the variables sepal length and width",
                 "and petal length and width, reScatter_plotsectively,",
                 "for 50 flowers from each of 3 Scatter_plotsecies of iris.",
                "The Scatter_plotsecies are Iris setosa, versicolor, and virginica.", sep = " ")
    text.p <- ggparagraph(text = text, face = "italic", size = 11, color = "black")
    

    组图展示,调整高度和宽度

    ggarrange(density.p, stable.p, text.p,
             ncol = 1, nrow = 3,
             heights = c(1, 0.5, 0.3))
    
    img

    子母图展示

    density.p + annotation_custom(ggplotGrob(stable.p),
                                 xmin = 5.5, ymin = 0.7,
                                 xmax = 8)
    
    img

    6)嵌套布局展示

    p1 <- ggarrange(Scatter_plots, Bar_plot + font("x.text", size = 9),
                   ncol = 1, nrow = 2)
    p2 <- ggarrange(density.p, stable.p, text.p,
                   ncol = 1, nrow = 3,
                   heights = c(1, 0.5, 0.3))
    #先组合P1,P2,然后自定义行 列 ,嵌套组合展示
    ggarrange(p1, p2, ncol = 2, nrow = 1)
    
    img

    参考链接:

    http://www.sthda.com/english/articles/24-ggpubr-publication-ready-plots/81-ggplot2-easy-way-to-mix-multiple-graphs-on-the-same-page/

    【公众号对话框,回复 R组图 即可获得上述组图R代码】

    更多关于生信,R,Python的内容请扫码关注小号,谢谢。


    img

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