系列回顾:
1.RNA velocity分析练习(一)文件下载以及预处理
要获取我们需要的loom文件,你需要先安装以下文件:
- 首先要求你的python >=3.6.0
- 安装依赖软件:
$ conda install numpy scipy cython numba matplotlib scikit-learn h5py click
当然你也可以选择用pip进行安装:
$ pip install numpy scipy cython numba matplotlib scikit-learn h5py click
- 安装pysam
$ pip install pysam
- 安装velocyto(这就是我们之后生成loom文件需要的软件)
$ pip install velocyto
- 测试是否安装成功:
$ velocyto --help
Usage: velocyto [OPTIONS] COMMAND [ARGS]...
Options:
--version Show the version and exit.
--help Show this message and exit.
Commands:
run Runs the velocity analysis outputting a loom file
run10x Runs the velocity analysis for a Chromium Sample
run_dropest Runs the velocity analysis on DropEst preprocessed data
run_smartseq2 Runs the velocity analysis on SmartSeq2 data (independent bam file per cell)
tools helper tools for velocyto
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