GO KEGG画图

作者: KK_f2d5 | 来源:发表于2019-05-22 16:16 被阅读2次

    之前的步骤参考:
    https://www.jianshu.com/p/aeffb9791572
    用David得到了数据,接下来画几张美丽的图。GO有三个部分,BP,CC, MF。一般我们使用BP。但是这里我们想把三个部分都展示在图片当中。

    rm(list=ls())
    library(ggplot2)
    library(Cairo)
    library(grid)
    
    GO_BP <- read.table("Term.txt",header = T,sep="\t")
    GO_BP <- within(GO_BP,{Term <- factor(Term, levels = GO_BP$Term)})
    tiff(filename = "GO_up1.tiff",
         width = 3000, height = 2000, units = "px",res = 300,compression = "lzw")
    plot <- ggplot(data=GO_BP)+
      geom_bar(aes(x=Term,y=Count, fill=-log10(PValue)), stat='identity') + 
      coord_flip() +
      scale_fill_gradient(expression(-log["10"](italic(p))),low="red", high = "blue") +
      scale_y_continuous(expand=c(0, 0), limits=c(0,12), breaks = c(seq(0, 12, 2)))+ 
      theme(
        panel.background = element_rect(fill = "transparent",colour = NA), 
       # panel.border = element_blank(),
       # panel.grid.minor = theme_blank(),
        plot.margin=unit(rep(1,4), 'lines'),
        #panel.grid.minor = element_line(colour = "black"),
      #  panel.grid.major = element_line(color = "black"),
        axis.line.y = element_line(colour = "black"),
        axis.line.x = element_line(colour = "black"),
        axis.title.x = element_text(size=12,color='black',face='bold'),
        axis.title.y = element_text(size=12,color='black',face='bold'),
        axis.text.x = element_text(size=12,color='black',face='bold'),
        axis.text.y = element_text(size=12,color='black',face='bold'),
        legend.text=element_text(color="black",size=rel(1.0)),
        legend.title = element_text(color="black",size=rel(1.1)),
        legend.position=c(0.8,0.4)
        # legend.position="top",
      )  +  annotate('segment', x=1, xend=7, y=3.2, yend=3.2, col = "gray", size = 1) + 
      annotate('segment', x=1, xend=1, y=3.0, yend=3.2, col = "gray", size = 1) + 
      annotate('segment', x= 7, xend=7, y=3.0, yend=3.2, col = "gray", size = 1) + 
      annotate("text",x=4, y = 4, label= "Biological process", hjust = 0.25, col = "black", size = 4) +
      annotate('segment', x=8, xend=10, y=9.2, yend=9.2, col = "gray", size = 1) + 
      annotate('segment', x=8, xend=8, y=9, yend=9.2, col = "gray", size = 1) + 
      annotate("text",x=9, y = 10, label= "Cellular component", hjust = 0.25, col = "black", size = 4) +
      annotate("text",x=11, y = 3.5, label= "Molecular function",  col = "black", size = 4) 
    
    image.png

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