转录组原始数据提交SRA数据库

点击NCBI主页Submit 点击SRA下Submit 点击New submission

在第4步BIOSAMPLE TYPE 选择Model organism or animal

BIOSAMPLE TYPE

在第5步BIOSAMPLE ATTRIBUTES需要注意，每个样本的所有属性不能与其他任何一个样本的属性完全一致，否则会出现报错。

Error:Your table upload failed because multiple BioSamples cannot have identical attributes. You should have one BioSample for each specimen, and each of your BioSamples must have differentiating information (excluding sample name, title, bioproject accession and description). This check was implemented to encourage submitters to include distinguishing information in their samples. If the distinguishing information is in the sample name, title or description, please recode it into an appropriate attribute, either one of the predefined attributes or a custom attribute you define. If it is necessary to represent true biological replicates as separate BioSamples, you might add an 'aliquot' or 'replicate' attribute, e.g., 'replicate = biological replicate 1', as appropriate. Note that multiple assay types, e.g., RNA-seq and ChIP-seq data may reference the same BioSample if appropriate.

添加replicate属性

使用ascp上传数据
ascp安装及添加到环境变量见：Windows安装Aspera和sratoolkit - 简书 (jianshu.com)
当然，不添加到环境变量也可以，需要每次使用前，使用cd命令切换到ascp的安装目录。